is a soft rot phytopathogenic bacterium mainly infecting potatoes. The virulence of is controlled by quorum sensing (QS), a communication mechanism which enables bacteria to coordinate their behavior in a population density-dependent manner. Inhibiting QS has gained interest as a sustainable alternative to conventional treatments to control pathogens in agriculture. Here, we investigate the potential of a robust lactonase to inhibit virulence in vitro, combining phenotypic and proteomic studies. We report that exogenous lactonase treatment reduced the secretion of pectate lyase, cellulase, and polygalacturonase enzymes, leading to decreased virulence on potato tubers. Major changes in the proteome revealed that lactonase affects the abundance of proteins with various functions, including virulence. Taken as a whole, these results suggest that lactonase-mediated disruption of QS in is a promising strategy to limit infections.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.4c07881 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metagenomic Sequencing of Tomato Plants with Wilt Symptoms Allows for Strain-Level Pathogen Identification and Genome-Based Characterization.
Phytopathology
January 2025
Virginia Polytechnic Institute and State University, School of Plant and Environmental Science, Blacksburg, Virginia, United States;
Diseases that affect the vascular system or the pith are of great economic impact since they can rapidly destroy the affected plants, leading to complete loss in production. Fast and precise identification is thus important to inform containment and management, but many identification methods are slow because they are culture-dependent and they do not reach strain resolution. Here we used culture-independent long-read metagenomic sequencing of DNA extracted directly from stems of two tomato samples that displayed wilt symptoms.
View Article and Find Full Text PDFMultistate kinetics of the syringe-like injection mechanism of Tc toxins.
Sci Adv
January 2025
Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
Tc toxins are pore-forming virulence factors of many pathogenic bacteria. Following pH-induced conformational changes, they perforate the target membrane like a syringe to translocate toxic enzymes into a cell. Although this complex transformation has been structurally well studied, the reaction pathway and the resulting temporal evolution have remained elusive.
View Article and Find Full Text PDFUbiquitination of OsCSN5 by OsPUB45 activates immunity by modulating the OsCUL3a-OsNPR1 module.
Sci Adv
January 2025
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
The COP9 signalosome (CSN) is a highly conserved protein complex in eukaryotes, with CSN5 serving as its critical catalytic subunit. However, the role of CSN5 in plant immunity is largely unexplored. Here, we found that suppression of in rice enhances resistance against the fungal pathogen and the bacterial pathogen pv.
View Article and Find Full Text PDFPtpA protein from Mycobacterium avium subsp. paratuberculosis as a potential marker of rheumatoid arthritis in humans.
PLoS One
January 2025
Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud Universidad de Guadalajara, Guadalajara, Mexico.
Studies have noted the connection between Mycobacterium avium subspecies paratuberculosis (MAP) and autoimmunity. MAP is an intracellular pathogen that infects and multiplies in macrophages. To overcome the hostile environment elicited by the macrophage, MAP secretes a battery of virulence factors to neutralize the toxic effects of the macrophage.
View Article and Find Full Text PDFExtracellular phospholipase, protease and hemolysin production by strains of opportunistic yeasts from the excreta of Mew Gulls breeding in natural and urban habitats.
Vet Res Commun
January 2025
Soil Science Faculty, Lomonosov Moscow State University, Moscow, 119234, Russia.
Extracellular hydrolytic activity (phospholipase, protease and hemolysin production) was evaluated in 178 strains of potentially pathogenic ascomycetous (Candida parapsilosis, Candida tropicalis) and basidiomycetous (Rhodotorula mucilaginosa) yeasts isolated from the excreta of Mew Gulls. Two bird colonies, one nesting in a natural habitat and the other in an urban habitat at the landfill, were studied simultaneously during their 7-month breeding season. Significant differences in phospholipase and protease production were found between natural and anthropophized strains.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!