We introduce a concept of aberration correction under microscopy that is based on observation of circular Brownian motion of an object driven by orbital angular momentum of a Laguerre-Gaussian (LG) beam. Following the concept, we establish an aberration-correction scheme by using a holographic optical tweezers setup equipped with a spatial light modulator that produces the LG beam as well as corrects the light wavefront. The light wavefront is modified adaptively to improve circular symmetry and uniformity of the orbit of a colloidal dielectric sphere revolving in mid-water under the irradiation of the LG beam. We reveal that the proposed scheme is sensitive to tiny phase difference of less than the accuracy of a highest-grade optical flat, 0.05λ, and is applicable to aberrations of up to the first 21 terms of the Zernike series expansion. The scheme not only improves the quality of optical tweezers but also enables to distinguish individual objective lenses assigned a common product code from difference in aberration-correction patterns. The present contribution therefore provides a useful tool for microscopy and laser fabrication in addition to the immediate application to optical trapping.
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http://dx.doi.org/10.1364/OE.528555 | DOI Listing |
Biophys Physicobiol
September 2024
Department of Cell Biology, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
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January 2025
Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei, China.
The spin angular momentum (SAM) plays a significant role in light-matter interactions. It is well known that light carrying SAM can exert optical torques on micro-objects and drive rotations, but 3D rotation around an arbitrary axis remains challenging. Here, we demonstrate full control of the 3D optical torque acting on a trapped microparticle by tailoring the vectorial SAM transfer.
View Article and Find Full Text PDFJ Med Life
November 2024
Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Optical tweezers, which leverage the forces exerted by radiation pressure, have emerged as a pivotal technique for precisely manipulating and analyzing microscopic particles. Since Arthur Ashkin's ground-breaking work in the 1970s and the subsequent development of the single-beam optical trap in 1986, the capabilities of optical tweezers have expanded significantly, enabling the intricate manipulation of biological specimens at the micro- and nanoscale. This review elucidates the foundational principles of optical trapping and their extensive applications in the biomedical sciences.
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January 2025
CNR Istituto Officina Dei Materiali, Area Science Park Basovizza, S.S. 14, Km 163,5, 34149, Trieste, Italy.
The organization and dynamics of the spectrin-actin membrane cytoskeleton play a crucial role in determining the mechanical properties of red blood cells (RBC). RBC are subjected to various forces that induce deformation during blood microcirculation. Such forces also regulate membrane tension, leading to Piezo1 channel activation, which is functionally linked to RBC dehydration through calcium influx and subsequent activation of Gardos channels, ultimately resulting in variations in RBC volume.
View Article and Find Full Text PDFMicromachines (Basel)
December 2024
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
The electric fields of tightly focused laser beams can be strong enough to apply appreciable force to microscopic objects, including biological entities such as cells, bacteria, and even viruses and biomolecules [...
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