AI Article Synopsis
- - MultiPerturb-seq is a new CRISPR screening platform that combines single-nucleus chromatin accessibility, transcriptome analysis, and guide RNA capture to enhance the profiling of genetic information.
- - This high-throughput method utilizes advanced techniques like combinatorial indexing and droplet microfluidics to efficiently process and integrate multiple forms of data from single cells.
- - The research identified critical differentiation genes in a rare pediatric cancer, highlighting ZNHIT1 as a promising target for developing reprogramming therapies for cancer treatment.
Article Abstract
Pooled single-cell CRISPR screens have profiled either gene expression or chromatin accessibility but not both modalities. Here we develop MultiPerturb-seq, a high-throughput CRISPR screening platform with joint single-nucleus chromatin accessibility, transcriptome and guide RNA capture using combinatorial indexing combined with droplet microfluidics to scale throughput and integrate all three modalities. We identify key differentiation genes in a rare pediatric cancer and establish ZNHIT1 as a potential target for cancer reprogramming therapy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/s41587-024-02475-x | DOI Listing |
Publication Analysis
Top Keywords
chromatin accessibility
12
crispr screens
8
joint single-nucleus
8
single-nucleus chromatin
8
accessibility transcriptome
8
pooled crispr
4
screens joint
4
transcriptome profiling
4
profiling pooled
4
pooled single-cell
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!