AI Article Synopsis
- This study investigates how phosphoglycerate mutase 1 (PGAM1) influences aerobic glycolysis and tumor aggressiveness in melanoma, suggesting it could be a target for new therapies.
- Researchers found that high levels of PGAM1 are associated with poor patient outcomes, as its silencing in melanoma cells reduced their proliferation, invasion, and glycolysis.
- The study concludes that PGAM1 plays a critical role in melanoma progression through its effects on apoptosis and immune response, highlighting potential pathways for therapeutic intervention.
Article Abstract
Background: The aim of this study was to investigate the mechanism through which phosphoglycerate mutase 1 (PGAM1) drives aerobic glycolysis and promotes tumor aggressiveness in melanoma and to evaluate its potential as a therapeutic target.
Methods: The survival of patients with skin cutaneous melanoma was predicted. The expression of PGAM1 in melanoma cells was measured, and then the levels of markers related to apoptosis, glycolysis and immune responses in melanoma cells cultured with or without CD8 T cells were detected. The effects of PGAM1 knockdown on the malignant phenotypes and extracellular acidification rate (ECAR) of melanoma cells were analyzed.
Results: High-expressed PGAM1 was predictive of a poor prognosis of melanoma. The viability, proliferation and invasion as well as glycolysis of PGAM1-silenced melanoma cells were suppressed. PGAM1 silencing lowered the expressions of glycolysis-related markers and anti-apoptosis marker BCL2 but promoted that of BAX, a pro-apoptosis marker. Downregulated levels of immune responses-related markers were observed after PGAM1 knockdown. POMHEX, a glycolysis inhibitor, reduced glycolysis and suppressed the levels of immune responses-related markers and anti-apoptosis marker BCL2.
Conclusion: This study revealed the involvement of PGAM1 in the aerobic glycolysis of melanoma, providing novel insights into the molecular mechanisms of melanoma progression.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.137898 | DOI Listing |
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