AI Article Synopsis
- This study presents a new UHPLC-HRMS method for quickly identifying intact bovine milk proteins after simulated digestion, distinguishing between modified and intact proteins.
- The method successfully detected differences in protein profiles of raw versus heated milk, revealing additional signals for non-enzymatic modifications in heated samples.
- The findings showcase the effectiveness of UHPLC-HRMS in analyzing protein structures, offering insights into how digestion impacts milk protein composition.
Article Abstract
This study demonstrates the development and application of an ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS) method for the rapid and sensitive identification of intact bovine milk proteins following simulated gastrointestinal infant digestion. The new method enables the differentiation between partially hydrolysed/modified and fully intact proteins. In the raw milk, intact α-lactalbumin was visible on SDS - PAGE until the end of the gastrointestinal digestion, while it was not detected with UHPLC-HRMS. Analysis of both raw and heated milk samples revealed that the method is applicable to various milk types. Interestingly, heated milk showed additional signals in the mass spectrum, indicating non-enzymatic post-translational modifications. The relative abundance of these proteoforms could be followed along digestion. These findings highlight the versatility and sensitivity of UHPLC-HRMS in elucidating protein structures and modifications, providing valuable insights into how simulated digestion affects milk protein composition.
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| http://dx.doi.org/10.1016/j.foodchem.2024.142034 | DOI Listing |
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