Thymoquinone loaded nanoemulgel in streptozotocin induced diabetic wound.

To treat diabetic wound healing with a novel Thymoquinone (TQ) loaded nanoformulation by using combination of essentials oils. TQ nanoemulsion (NE) was developed with seabuckthorn & lavender essential oils by phase inversion method and mixture design. Further, DIAGEL is obtained by incorporating NE into 1% carbopol934. Furthermore, particle size, polydispersity index, thermodynamic stability studies, rheology, spreadability, drug content, drug release, permeation, anti-oxidant assay, antimicrobial studies, angioirritance, HAT-CAM assay, and studies were determined. NE has a particle size of 17.79 ± 0.61 nm, 0.206 ± 0.012 PDI & found to be thermodynamically stable. DIAGEL exhibited pseudoplastic behavior, sustained drug release, better permeation of TQ and a drug content of 98.54 ± 0.08%. DIAGEL stored for 6 months at room temperature and 2-8°C showed no degradation. Further, an improved angiogenesis, absence of angio-irritancy, remarkable antioxidant and antimicrobial activities against & were observed. Cytotoxicity analysis revealed nearly 2.28 -folds higher IC50 value than drug solution. Furthermore, inflammatory mediators were reduced in DIAGEL treated animal groups. The histopathological studies confirmed skin healing with regeneration and granulation of tissue. The novel formulation has strong anti-inflammatory, angiogenesis, antioxidant and appreciable diabetic wound healing properties.