AI Article Synopsis
- Research on mRNA stability in wheat, a crucial crop, is limited, so we studied mRNA decay in durum wheat to understand its regulatory landscape.
- Our findings show that the structure of the 3' UTR and preference for RNA motifs across subgenomes affect mRNA stability and abundance, creating an imbalance in decay among subgenomic RNAs.
- Additionally, variations in RNA structural motifs due to domestication can lead to differences in mRNA stability and expression levels, highlighting the significance of RNA structure in gene regulation and potential crop improvement strategies.
Article Abstract
Despite the critical role of mRNA stability in post-transcriptional gene regulation, research on this topic in wheat, a vital agricultural crop, remains unclear. Our study investigated the mRNA decay landscape of durum wheat (Triticum turgidum L. ssp. durum, BBAA), revealing subgenomic asymmetry in mRNA stability and its impact on steady-state mRNA abundance. Our findings indicate that the 3' UTR structure and homoeolog preference for RNA structural motifs can influence mRNA stability, leading to subgenomic RNA decay imbalance. Furthermore, single-nucleotide variations (SNVs) selected for RNA structural motifs during domestication can cause variations in subgenomic mRNA stability and subsequent changes in steady-state expression levels. Our research on the transcriptome stability of polyploid wheat highlights the regulatory role of non-coding region structures in mRNA stability, and how domestication shaped RNA structure, altering subgenomic mRNA stability. These results illustrate the importance of RNA structure-mediated post-transcriptional gene regulation in wheat and pave the way for its potential use in crop improvement.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579497 | PMC |
| http://dx.doi.org/10.1038/s41467-024-54172-7 | DOI Listing |
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