AI Article Synopsis
- FOXO1 is an important transcription factor involved in key cellular processes like cell differentiation, apoptosis, and metabolism, making it a focus for research.
- The chapter discusses using CRISPR exon scanning as a novel method to explore functional domains of FOXO1, aiming to clarify its regulatory roles.
- This method helps identify crucial areas of the FOXO1 gene and provides insights into how different regions impact its functions in various biological scenarios.
Article Abstract
The study of FOXO1, a pivotal transcription factor, has garnered significant attention due to its critical role in diverse cellular processes, including lineage differentiation, apoptosis, cell cycle regulation, and metabolism. To comprehensively understand the functional intricacies of FOXO1, an innovative approach is essential. This chapter highlights employing CRISPR exon scanning as a strategic tool to dissect the functional domains of FOXO1 and unravel its diverse regulatory functions. CRISPR exon scan allows for the identification of functionally important domains based on the levels of sgRNA depletion or enrichment within the FOXO1 gene, providing a unique opportunity to investigate the domain function under relevant biological contexts. This approach enables the systematic exploration of FOXO1's structural domains, shedding light on how distinct regions contribute to its overall function. The comprehensive exon scan analysis using CRISPR technology allows gaining a nuanced understanding of FOXO1's functional diversity and regulatory mechanisms.
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| http://dx.doi.org/10.1007/978-1-0716-4217-7_6 | DOI Listing |
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