The present study evaluated the isolates obtained from various origins in Iran. strains previously recovered and stored in the veterinary microbiology laboratory were serotyped and subjected to antibiotic susceptibility test, detection of the virulence and resistance genes by polymerase chain reaction (PCR), and genotyping by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). All isolates showed resistance to erythromycin and the most resistance rates were detected for trimethoprim (86.66%), ampicillin (75.00%), and sulfamethoxazole-trimethoprim (63.33%), respectively. In total, 86.33% of the isolates were known as multi-drug resistant and none of the isolates showed resistance to cefepime, nalidixic acid, imipenem, ceftriaxone, and polymyxin B. The virulence genes, , , and besides the resistance gene were identified in all 60 strains. The most prevalent resistance genes were respectively (70.00%), (58.33%), and (55.00%). Statistical analysis revealed a significant difference between serotypes associated with the resistance gene. In ERIC-PCR analysis, 14 distinct clusters were obtained. Statistically, there were significant relationships between the source and ERIC's genomic pattern and between the serotype of isolates and genotypic pattern of ERIC. According to the results, serotypes from non-human sources had considerable resistance to different antibiotics and carried significant virulence determinants and resistance genes. In addition, ERIC-PCR showed relevant results in discriminating serotypes from other sources.
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| http://dx.doi.org/10.30466/vrf.2024.2019507.4123 | DOI Listing |
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