Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11581815 | PMC |
| http://dx.doi.org/10.5468/ogs.24019.e1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chloroquine inhibits artificial oocyte activation induced by ethanol or Sr²⁺ but not by sperm in mice.
J Reprod Dev
December 2024
Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi, Yamanashi 400-0016, Japan.
Calcium release from the endoplasmic reticulum via sperm-derived phospholipase C zeta is crucial for oocyte activation during fertilization. Chloroquine (CQ) inhibits the increase in cytoplasmic calcium. This study investigated the effects of CQ on fertilization and oocyte activation.
View Article and Find Full Text PDFZwitterionic, Stimuli-Responsive Liposomes for Curcumin Drug Delivery: Enhancing M2 Macrophage Polarization and Reducing Oxidative Stress through Enzyme-Specific and Hyperthermia-Triggered Release.
ACS Appl Bio Mater
December 2024
Institute of Physics - Centre for Science and Education, Silesian University of Technology, Krasińskiego 8, Katowice 40-019, Poland.
A zwitterionic, stimuli-responsive liposomal system was meticulously designed for the precise and controlled delivery of curcumin, leveraging enzyme-specific and hyperthermic stimuli to enhance therapeutic outcomes. This platform is specifically engineered to release curcumin in response to , an enzyme that degrades phospholipids, enabling highly targeted and site-specific drug release. Mild hyperthermia (40 °C) further enhances membrane permeability and activates thermosensitive carriers, optimizing drug delivery.
View Article and Find Full Text PDFPhospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic development.
Obstet Gynecol Sci
November 2024
Reproductive Medicine, Developmental and Reproductive Biology, Regional University Hospital & School of Medicine and Peritox Laboratory, Picardie University Jules Verne, Amiens, France.
Voltage- and Ca-inducible PLC activity for analyzing PI(4,5)P sensitivity of ion channels in Xenopus oocytes.
Biochim Biophys Acta Biomembr
January 2025
Graduate School of Medicine, Osaka University, Japan; Graduate School of Frontier Biosciences, Osaka University, Japan.
Phosphatidylinositol 4,5-bisphosphate (PIP) is a key membrane lipid regulating various ion channel activities. Currently, several molecular tools are used to modulate PIP levels, each of which has distinct advantages and drawbacks. In this study, we proposed a novel methodology using heterologous Xenopus oocytes to precisely manipulate PIP levels using phospholipase C (PLC)-ζ, which hydrolyzes PIP.
View Article and Find Full Text PDFArtificial oocyte activation improves ICSI outcomes following unexplained fertilization abnormalities.
Reprod Biomed Online
November 2024
Reproductive Medicine Research Group, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK; Assisted Conception Unit, Ninewells Hospital, Dundee, UK. Electronic address:
Research Question: Is artificial oocyte activation (AOA) effective for patients with unexplained low or no fertilization following IVF/intracytoplasmic sperm injection (ICSI)?
Design: All IVF/ICSI cases resulting in total fertilization failure or fertilization rate ≤25% at Ninewells Assisted Conception Unit, Dundee between January 2014 and December 2021 (n = 231) were reviewed contemporaneously. After exclusion of obvious stimulation, egg, sperm and/or assisted reproductive technology laboratory factors, patients with at least one cycle of IVF/ICSI resulting in apparently unexplained fertilization abnormalities were offered research investigations, including sperm immunocytochemistry for phospholipase C zeta (PLCζ) protein expression. This retrospective case-control cohort study evaluated laboratory and clinical outcomes for 39 couples (15 attended for sperm studies research) that subsequently undertook ICSI-AOA with Ca ionophore.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!