AI Article Synopsis
- - The study aimed to evaluate how effective nanopore sequencing technology is for diagnosing tuberculous meningitis (TBM) using cerebrospinal fluid samples from patients.
- - Compared to traditional methods like acid-fast staining and culture, nanopore sequencing had a sensitivity of 43.30% and an area under the ROC curve of 0.661, indicating better performance overall.
- - When nanopore sequencing was used alongside imaging techniques, it achieved the best diagnostic results with increased sensitivity, specificity, and predictive values, showing its potential as a reliable tool for TBM diagnosis.
Article Abstract
Objective: To investigate the diagnostic efficacy of nanopore sequencing technology in tuberculous meningitis (TBM).
Methods: Cerebrospinal fluid samples were collected from patients for acid-fast staining microscopy, Mycobacterium tuberculosis solid culture, DNA detection, and nanopore sequencing. Lastly, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the receiver operating characteristic curve (AUC) were calculated and compared among these detection methods.
Results: In this study, 30 TBM patients and 18 non-TBM patients were included. Nanopore sequencing showed higher sensitivity (43.30 %) and AUC (0.661) compared to the other methods. Combining nanopore sequencing and imaging achieved the highest diagnostic performance with sensitivity (60.00 %), specificity (88.90 %), PPV (90.00 %), NPV (57.10 %), and AUC (0.744).
Conclusion: Nanopore sequencing demonstrated superior diagnostic accuracy for TBM, outperforming acid-fast staining, Mycobacterium tuberculosis culture, and DNA detection. When combined with imaging, nanopore sequencing significantly enhanced diagnostic sensitivity and accuracy for TBM.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2024.116593 | DOI Listing |
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