Differentiation protocols used to generate stem cell-derived islet cells yield heterogenous cell populations. We generated a human embryonic stem cell line that reports insulin- and glucagon-expressing cells in vitro and in vivo without altering their differentiation or function. We showed some insulin- and glucagon-expressing bihormonal cells are cell-autonomously fated to become α-like cells. This reporter cell line can be used to further study and improve stem cell-derived islet differentiation and transplantation.
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| http://dx.doi.org/10.2337/db24-0756 | DOI Listing |
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BC Children's Hospital Research Institute, University of British Columbia, Vancouver, British Columbia, Canada.
Differentiation protocols used to generate stem cell-derived islet cells yield heterogenous cell populations. We generated a human embryonic stem cell line that reports insulin- and glucagon-expressing cells in vitro and in vivo without altering their differentiation or function. We showed some insulin- and glucagon-expressing bihormonal cells are cell-autonomously fated to become α-like cells.
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Division of Developmental Biology, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati OH 45229, USA; Center for Stem Cell and Organoids Medicine (CuSTOM), CCHMC, Cincinnati OH 45229, USA; Division of Endocrinology, CCHMC, Cincinnati OH 45229, USA. Electronic address:
Human pluripotent stem cells (hPSCs) represent an unlimited source of β-like cells for both disease modeling and cellular therapy for diabetes. Numerous protocols have been published describing the differentiation of hPSCs into β-like cells that secret insulin in response to a glucose challenge. However, among the most widely used protocols it is not clear which yield the most functional cells with reproducible glucose-stimulated insulin-secretion (GSIS).
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