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Involvement of canine parvovirus in mRNA expression levels of key lectins and caspases in blood leukocytes. | LitMetric

Involvement of canine parvovirus in mRNA expression levels of key lectins and caspases in blood leukocytes.

Vet Res Commun

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Published: November 2024

AI Article Synopsis

  • * A study examined the immune response and blood cells in dogs infected with CPV-2, finding significant changes in certain blood indices but not in apoptosis markers, suggesting a different mechanism for immunosuppression.
  • * The study highlighted the roles of immune molecules galectin-1 and Mincle, indicating they may help the body defend against CPV-2 by affecting viral adhesion, but more research is needed to understand their functions.

Article Abstract

Canine parvovirus disease (CPVD) is one of the most common causes of viral diarrhea in dogs. The disease has a mortality rate of up to 90% if left untreated, and can cause gastroenteritis, vomiting, mucoid/hemorrhagic diarrhea, lymphopenia and even immunosuppression. Based on the effects of canine parvovirus type 2 (CPV-2) on the immune system, we investigated the effects of the CPV-2 on hematological indices and the expression of certain immune molecules in blood leukocytes of CPV-positive and non-CPV dogs. The dogs were (para)clinically evaluated, and their disease status was confirmed by antigen rapid detection kits and polymerase chain reaction (PCR). To elucidate the nature of the immunosuppression seen in CPVD, we investigated the expression of caspases 3/7 and 9, and some lectin family molecules such as galectin-1 (important in viral adhesion) and Mincle (macrophage‑inducible C‑type lectin receptor), in blood leukocytes using reverse transcription quantitative PCR (RT-qPCR). We observed remarkable lymphopenia, lower Hb concentration and higher red blood cell distribution width (RDW) value in CPV-positive dogs. No significant changes in expression of caspases 3/7 and 9 were detected, but galectin-1 and Mincle showed remarkable down and up-regulation, respectively. This proves that the immunosuppression seen in most CPVD is not caused by lymphocyte apoptosis in blood, and that Mincle is partially involved in the immune response to CPV-2. The observed changes in galectin-1 and Mincle may be a defense mechanism against parvovirus by potentially preventing the parvovirus from adhering to the cells. Further research is, nonetheless, needed to elucidate the possible roles of these molecules in CPV-2 pathogenesis and the immune system's response to parvovirus.

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Source
http://dx.doi.org/10.1007/s11259-024-10586-8DOI Listing

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