Hemostatic function, immunomodulatory capacity, and effects of lipemia in cold-stored whole blood.

Transfusion

Research and Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia.

Published: November 2024

AI Article Synopsis

  • Whole blood (WB) is being utilized more for trauma resuscitation, but its biological response modifiers (BRMs) during storage have not been extensively studied.
  • This study involved collecting WB and examining the quality and function of its components, finding significant reductions in leukocytes and variations in platelet recovery over a 21-day cold storage period.
  • Results indicate WB maintains hemostatic function despite some quality issues (like lipemia) and emphasizes the need for quality control measures before it can be approved for transfusion use in Australia.

Article Abstract

Background: Whole blood (WB) is increasingly being used for resuscitation of trauma patients. Although platelet-, red blood cell (RBC)- and plasma-specific parameters in cold-stored WB are well characterized, there has been limited investigation of biological response modifiers (BRMs), which may induce adverse reactions in recipients. The aim of this study was to evaluate the quality and function of RBC, platelets, plasma proteins, and BRMs in cold-stored WB during storage.

Methods: WB (n = 24) was collected into collected into citrate-phosphate-dextrose (CPD) anticoagulant, held overnight, processed through a platelet-sparing filter, and stored at 2-6°C for 21 days. RBC, platelet, coagulation factor quality and function, and BRM concentrations were measured throughout the duration of storage.

Results: WB was effectively leukoreduced, with 99.98% reduction in leukocyte count and 81% platelet count recovery following filtration. Five WB units were significantly lipemic, with a visible lipid layer appearing after being cold storage overnight. These were more turbid with higher hemolysis compared to non-lipemic units (p = .023). Despite a decrease in platelet count during storage (p < .001), hemostatic function as measured by thromboelastography was maintained for at least 21 days (R time and maximum amplitude; both p < .001). There was a significant increase in PF4, CD62P, and RANTES during cold storage (all p < .001).

Discussion: WB retains hemostatic potential for at least 21 days of cold storage, and with further development, may be suitable for transfusion in Australia. Before implementation in Australia, quality control measures for lipemia and hemolysis would need to be defined as part of our manufacturing processes.

Download full-text PDF

Source
http://dx.doi.org/10.1111/trf.18065DOI Listing

Publication Analysis

Top Keywords

quality function
8
platelet count
8
hemostatic function
4
function immunomodulatory
4
immunomodulatory capacity
4
capacity effects
4
effects lipemia
4
lipemia cold-stored
4
cold-stored blood
4
blood background
4

Similar Publications

Background: Infantile colic (IC) is a functional gastrointestinal disorder characterized by crying spells lasting more than 3 hours a day, more than 3 days a week, for 3 weeks, according to the Rule of Three's.

Objective: This study aimed to evaluate the effect of added probiotic supplementation in the mother's diet on the crying frequency of babies with infantile colic, maternal attachment, and maternal quality of life.

Design And Settings: 36 mothers and their babies were randomized into two groups.

View Article and Find Full Text PDF

Purpose: Although germline genetic testing (GT) is recommended for all patients with ovarian cancer (OC) and some patients with endometrial cancer (EC), uptake remains low with multiple barriers. Our center performs GT in parallel with somatic testing via a targeted sequencing assay (MSK-IMPACT) and initiates testing in oncology clinics (mainstreaming). We sought to optimize our GT processes for OC/EC.

View Article and Find Full Text PDF

Background And Objectives: Three phase 3 trials demonstrated the efficacy and safety of atogepant in episodic migraine (EM) and chronic migraine (CM) across 12-week treatment periods. This analysis evaluates improvements in efficacy and functional outcomes in the first 4 weeks of treatment with the oral calcitonin gene-related peptide receptor antagonist, atogepant, for the preventive treatment of migraine.

Methods: ADVANCE, ELEVATE, and PROGRESS were phase 3, multicenter, randomized, double-blind, placebo-controlled 12-week trials.

View Article and Find Full Text PDF

The cashmere goat is a type of livestock primarily known for its cashmere. Cashmere has a soft hand feel and good luster. It is a vital raw material in the textile industry, possessing significant economic value.

View Article and Find Full Text PDF

Outcomes for Younger Patients with Femoral Neck Fractures.

J Bone Joint Surg Am

December 2024

Edinburgh Orthopaedics, Royal Infirmary of Edinburgh, Edinburgh, Scotland.

Background: There is a paucity of longer-term outcome data in younger adult patients who undergo fixation for an intracapsular hip fracture. The aims of this study were to evaluate the outcomes for young adult patients undergoing intracapsular hip fracture fixation and to assess factors associated with failure and patient-reported outcome measures (PROMs).

Methods: From 2008 to 2018, 112 consecutive patients ≤60 years of age (mean age, 48 years [range, 20 to 60 years]; 54% male) were retrospectively identified as having undergone fixation of an intracapsular hip fracture.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: