AI Article Synopsis
- Gongju, a traditional Chinese medicinal herb, contains chlorogenic acid (CGA), and the study explores the increased CGA levels in autooctoploid Gongju compared to its parental tetraploid variety across different flowering stages.* -
- The research utilized integrated transcriptome and metabolome analyses to identify the molecular mechanisms affecting CGA biosynthesis, revealing significant differences in gene expression and metabolite accumulation between the two varieties at budding, early flowering, and full flowering stages.* -
- Key findings included the discovery that specific enzymes and transcription factors crucial for CGA synthesis were more actively expressed in octoploid Gongju compared to tetraploid, indicating their role in the enhanced CGA production during flowering.*
Article Abstract
Background: Gongju is recognized as one of the four traditional Chinese medicinal herbs, and its main constituents are chlorogenic acid (CGA) and its derivative material. CGA content in autooctoploid Gongju are considerably elevated compared with those in parental tetraploid Gongju at different flowering stages. However, the underlying molecular mechanisms governing the regulation CGA content remain poorly understood.
Methods: Therefore, we conducted integrated transcriptome and metabolome analyses of different flowering stages in autooctoploid and tetraploid Gongju to elucidate the underlying molecular mechanisms governing CGA biosynthesis.
Results: Transcriptome analysis showed that the number of differentially expressed genes in the budding stage (BS), early flowering stage (EF), and full flowering stage (FF) of tetraploid and octoploid Gongju were 3859, 11,211, and 6837, respectively. A total of 563, 466, and 394 differential accumulated metabolites were respectively identified between the bud stages of tetraploid and octoploid Gongju (4BS vs. 8BS), between the early flowering stages of tetraploid and octoploid Gongju (4EF vs. 8EF), and the full flowering stages of tetraploid and octoploid Gongju (4FF vs. 8FF) groups. The integrated analysis of transcriptomics and metabolomics revealed that the expression of pma6460 and mws0178, which are key enzymes involved in the CGA synthesis pathway, increased during the flowering stages of octoploid Gongju relative to that of tetraploid Gongju. The expression levels of and genes associated with CGA synthesis were higher in octoploid plants than in tetraploid plants at various flowering stages. To investigate the potential regulation of transcription factors involved in CGA synthesis, we analyzed the coexpression of and with and . Results indicated that transcription factors, such as (Cluster-30519.0), (Cluster-75874.0), (Cluster-94106.0), (Cluster-71968.7), (Cluster-32024.1), (Cluster-62341.0), (Cluster-32024.8), (Cluster-60210.0), and (Cluster-90665.1) play a pivotal role in CGA synthesis regulation. The present study provides novel insights into the molecular mechanisms underlying CGA accumulation in autopolyploid Gongju.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563975 | PMC |
| http://dx.doi.org/10.3389/fpls.2024.1461357 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
GraphkmerDTA: integrating local sequence patterns and topological information for drug-target binding affinity prediction and applications in multi-target anti-Alzheimer's drug discovery.
Mol Divers
January 2025
Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases Ministry of Education, Jiangxi Province Key Laboratory of Biomaterials and Biofabrication for Tissue Engineering, Gannan Medical University, Ganzhou, 341000, Jiangxi, China.
Identifying drug-target binding affinity (DTA) plays a critical role in early-stage drug discovery. Despite the availability of various existing methods, there are still two limitations. Firstly, sequence-based methods often extract features from fixed length protein sequences, requiring truncation or padding, which can result in information loss or the introduction of unwanted noise.
View Article and Find Full Text PDFThe MADS-box protein SHATTERPROOF 2 regulates TAA1 expression in the gynoecium valve margins.
Plant Reprod
January 2025
Hormonal Crosstalk in Plant Development, Mendel Center for Plant Genomics and Proteomics, CEITEC MU-Central European Institute of Technology, Masaryk University, 625 00, Brno, Czech Republic.
SHATTERPROOF 2 regulates TAA1 expression for the establishment of the gynoecium valve margins. Gynoecium development and patterning play a crucial role in determining the ultimate structure of the fruit and, thus, seed production. The MADS-box transcription factor SHATTERPROOF 2 (SHP2) contributes to valve margin differentiation and plays a major role in fruit dehiscence and seed dispersal.
View Article and Find Full Text PDFIntegrative physiological, biochemical, and proteomic analysis of the leaves of two cotton genotypes under heat stress.
PLoS One
January 2025
Department of Biochemistry, Bahauddin Zakariya University, Multan, Pakistan.
Cotton (Gossypium hirsutum L.), a crucial global fibre and oil seed crop faces diverse biotic and abiotic stresses. Among these, temperature stress strongly influences its growth, prompting adaptive physiological, biochemical, and molecular changes.
View Article and Find Full Text PDFDementia Care Practice.
Alzheimers Dement
December 2024
University of the West of Scotland, Paisley, Scotland, United Kingdom.
My PhD research is about Young-Onset Dementia and Career Development, focusing on women's experiences. This artwork or abstract ('artstract') represents how the research has developed to date. Understanding of career is informed by Super's (1980) Life-Span, Life-Space approach to career development, which can be understood flexibly to incorporate diverse experiences (including those of women and those living with a dementia diagnosis during working age).
View Article and Find Full Text PDFIntraspecific and intra-individual chemodiversity and phenotypic integration of terpenes across plant parts and development stages in an aromatic plant.
Plant Biol (Stuttg)
January 2025
Chemical Ecology, Bielefeld University, Bielefeld, Germany.
Some plant species produce an extraordinary diversity of specialized metabolites. The diverse class of terpenes is characteristic for many aromatic plants, and terpenes can occur as both emitted volatiles and stored compounds. Little is known about how intraspecific chemodiversity and phenotypic integration of both emitted volatile and stored terpenes differ intra-individually across plant development and between different plant parts, and studies considering both spatial and temporal scales are scarce.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!