Background: Parasites secrete and excrete a variety of molecules evolve to help establish and sustain infections within hosts. Parasite adaptation to their host may lead to between-population divergence in these excretory and secretory products (ESPs), but few studies have tested for intraspecific variation in helminth proteomes.
Methods: is a cestode that parasitizes three spined stickleback, . We used an ultra-performance liquid chromatography-mass spectrometry protocol to characterize the ESP and whole-body proteome of S. solidus. Specifically, we characterized the proteome of S. solidus at the plerocercoid stage from wild caught stickleback from three lakes on Vancouver Island (British Columbia, Canada) and one lake in Alaska (United States). We tested for differences in proteome composition among the four populations and specifically between ESPs and body tissue.
Results: Overall, we identified 1362 proteins in the total proteome of S. solidus, with 542 of the 1362 proteins detected exclusively in the ESPs. Of the ESP proteins, we found signaling peptides and transmembrane proteins that were previously not detected or characterized in S. solidus. We also found protein spectrum counts greatly varied between all lake populations.
Conclusions: These population-level differences were observed in both ESP and tissue types. Our study suggests that S. solidus can excrete and secrete a wide range of proteins which are distinct among populations. These differences might reflect plastic responses to host genotype differences, or evolved adaptations by Schistocephalus to different local host populations.
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| http://dx.doi.org/10.1101/2024.10.25.618520 | DOI Listing |
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Background: Parasites secrete and excrete a variety of molecules evolve to help establish and sustain infections within hosts. Parasite adaptation to their host may lead to between-population divergence in these excretory and secretory products (ESPs), but few studies have tested for intraspecific variation in helminth proteomes.
Methods: is a cestode that parasitizes three spined stickleback, .
Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host.
Biol Open
November 2021
Department of Proteomic Research and Mass Spectrometry, Institute of Biomedical Chemistry (IBMC), 10 Pogodinskaya street, 119121 Moscow, Russia.
The protein composition of the cestode Schistocephalus solidus was measured in an experiment simulating the trophic transmission of the parasite from a cold-blooded to a warm-blooded host. The first hour of host colonisation was studied in a model experiment, in which sticklebacks Gasterosteus aculeatus infected with S. solidus were heated at 40°C for 1 h.
View Article and Find Full Text PDFThe parasite Schistocephalus solidus secretes proteins with putative host manipulation functions.
Parasit Vectors
August 2021
Département de Biologie, Université Laval, Quebec, QC, Canada.
Background: Manipulative parasites are thought to liberate molecules in their external environment, acting as manipulation factors with biological functions implicated in their host's physiological and behavioural alterations. These manipulation factors are part of a complex mixture called the secretome. While the secretomes of various parasites have been described, there is very little data for a putative manipulative parasite.
View Article and Find Full Text PDFZone of Interaction Between the Parasite and the Host: Protein Profile of the Body Cavity Fluid of Gasterosteus aculeatus L. Infected with the Cestode Schistocephalus solidus (Muller, 1776).
Acta Parasitol
June 2021
Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, 185910, Petrozavodsk, Russia.
Article Synopsis
- - The study aimed to identify the proteins involved in communication between the parasite Schistocephalus solidus and its intermediate host, the three-spined stickleback fish Gasterosteus aculeatus, during infection by analyzing excretory-secretory proteins from both organisms.
- - Using mass-spectrometry nLC-MS/MS, researchers compared protein samples from infected sticklebacks to those from uninfected counterparts, identifying significant changes in protein levels, including increases in immune-related proteins in infected fish.
- - The results highlighted a total of 215 identified proteins, with 30 specific to the parasite, indicating alterations in the immune response and protein composition due to the infection, emphasizing the role of these proteins in host-parasite
Identification of candidate mimicry proteins involved in parasite-driven phenotypic changes.
Parasit Vectors
April 2015
Institut de Biologie Intégrative et des Systèmes (IBIS), Département de Biologie, Université Laval, Pavillon Charles-Eugènes-Marchand, Québec, G1V 0A6, Canada.
Background: Endoparasites with complex life cycles are faced with several biological challenges, as they need to occupy various ecological niches throughout their development. Host phenotypes that increase the parasite's transmission rate to the next host have been extensively described, but few mechanistic explanations have been proposed to describe their proximate causes. In this study we explore the possibility that host phenotypic changes are triggered by the production of mimicry proteins from the parasite by using an ecological model system consisting of the infection of the threespine stickleback (Gasterosteus aculeatus) by the cestode Schistocephalus solidus.
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