AI Article Synopsis
- Numerous treatments for critical limb ischemia (CLI) have struggled in clinical trials, especially stem cell therapy, which generally sees low patient survival rates.
- This study explored how polydeoxyribonucleotide (PDRN) affects stem cells from human deciduous teeth (SHED), finding that PDRN boosts their growth, movement, antioxidant abilities, and energy production through Akt activation.
- PDRN-enhanced SHED not only survived better in an ischemic environment but also showed potential as a new therapeutic option for CLI, highlighting the importance of Akt in this process.
Article Abstract
Although numerous approaches have emerged to address the challenges of critical limb ischemia (CLI), their clinical trials have proven elusive. Stem cell therapy has been utilized for CLI; however, its efficacy is limited, resulting in low survival rates in patients. Here, we investigated the impact of polydeoxyribonucleotide (PDRN) on the bioactivities of stem cells derived from human exfoliated deciduous teeth (SHED) against oxidative stress. PDRN treatment increased the proliferation, migration, antioxidant properties, and mitochondrial respiration of SHED. These beneficial effects were regulated by Akt activation. Through a murine hindlimb ischemia model, PDRN treatment demonstrated augmented the survival and proliferation of transplanted SHED at ischemic injury sites, whereas the inhibition of Akt suppressed these effects. Our findings revealed that PDRN promoted the therapeutic potential of SHED via Akt phosphorylation, suggesting PDRN-primed SHED as promising candidates for the development of novel stem cell therapeutics.
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| http://dx.doi.org/10.1016/j.bbrc.2024.150947 | DOI Listing |
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