Syngas fermentation using acetogenic bacteria offers a promising route for sustainable chemical production. However, gas-liquid mass transfer limitations and efficient co-utilization of CO and H pose significant challenges. This study investigated the kinetics of syngas conversion to acetate by Acetobacterium wieringae and Clostridium species in batch conditions under varying initial CO partial pressures (19 - 110 kPa). A. wieringae strains, exhibited superior growth in all gas compositions, with a maximum growth rate of 0.104 h. The distinct CO, H, and CO consumption patterns revealed metabolic flexibility and adaptation to varying syngas compositions. Notably, A. wieringae strains and C. autoethanogenum achieved complete CO and H conversion, with C. autoethanogenum also exhibiting net CO uptake. These findings provide valuable insights into the distinct metabolic capabilities of these acetogens and contribute to the development of efficient and sustainable syngas fermentation processes.
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http://dx.doi.org/10.1016/j.biortech.2024.131823 | DOI Listing |
Appl Microbiol Biotechnol
December 2024
Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Syngas fermentation to ethanol has reached industrial production. Further improvement of this process would be aided by quantitative understanding of the influence of imposed reaction conditions on the fermentation performance. That requires a reliable model of the microbial kinetics.
View Article and Find Full Text PDFMolecules
November 2024
Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
The use of mixed cultures in gas fermentations could reduce operating costs in the production of liquid chemicals such as alcohols or carboxylic acids. However, directing reducing equivalents towards the desired products presents the challenge of co-existing competing pathways. In this study, two trickle bed reactors were operated at acetogenic and chain elongating conditions to explore the fate of electron equivalents (ethanol, H, and CO) and test pH oscillations as a strategy to target chain-elongated products.
View Article and Find Full Text PDFMicrob Biotechnol
December 2024
Institute of Process Engineering in Life Sciences 2: Electro Biotechnology, Karlsruhe Institute of Technology - KIT, Karlsruhe, Germany.
Pyrolysis of lignocellulosic biomass commonly produces syngas, a mixture of gases such as CO, CO and H, as well as an aqueous solution generally rich in organic acids such as acetate. In this study, we evaluated the impact of increasing acetate shock loads during syngas co-fermentation with anaerobic microbiomes at different pH levels (6.7 and 5.
View Article and Find Full Text PDFCurr Pollut Rep
November 2024
Swedish Centre for Resource Recovery, University of Borås, Borås, Sweden.
Synthesis gas (syngas) fermentation represents a promising biological method for converting industrial waste gases, particularly carbon monoxide (CO) and carbon dioxide (CO₂) from industrial sources (e.g. steel production or municipal waste gasification), into high-value products such as biofuels, chemicals, and animal feed using acetogenic bacteria.
View Article and Find Full Text PDFBioresour Technol
February 2025
Institute of Sustainable Processes, Doctor Mergelina s/n, Valladolid 47011, Spain; Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Doctor Mergelina s/n, Valladolid 47011, Spain. Electronic address:
Syngas from the gasification of organic wastes represents a promising feedstock for fostering a sustainable bioeconomy. However, its potential is currently constrained by the low-value products generated. Osmolytes, such as hydroxyectoine, are high-value compounds, however, their biological production as isolated osmolytes is not yet cost-effective.
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