AI Article Synopsis
- Lipases are important enzymes with unique selectivity, making them useful in industry, especially for creating high-value products like structured lipids.
- A new analytical method was developed using HPLC to effectively separate tricapryloylglycerol and its breakdown products, achieving impressive resolution and consistency in under 15 minutes.
- An interface-based kinetic model was created to better understand lipase selectivity during the breakdown of medium- and long-chain TAGs, providing improved results compared to previous models and enhancing knowledge for future lipid production.
Article Abstract
Lipases are essential enzymes with unique selectivity, making them valuable in industrial applications. Understanding the integral stereoselectivity of lipases during triacylglycerol (TAG) hydrolysis is crucial for producing high-value products, such as structured lipids. This study developed an analytical method and an interface-based kinetic model to determine integral stereoselectivities on tricapryloylglycerol (TCG), a medium-chain TAG. The analytical method used an HPLC system that simultaneously separated TCG and its hydrolysates with resolution factors of >2.4 and relative standard deviation of retention times <0.3 % within 15 min. The interface-based kinetic model was established to determine the integral stereoselectivities according to the characteristics of the reaction system. The model provided better fitting results for TCG and trioleoylglycerol hydrolysis than a previous model, indicating the successful application in both medium- and long-chain TAGs. This study expanded our understanding of integral stereoselectivity and could facilitate the development of various structured lipid syntheses.
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| http://dx.doi.org/10.1016/j.foodchem.2024.141403 | DOI Listing |
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Interface-based kinetic model considering the integral stereoselectivity of lipases on tricapryloylglycerol in a reverse micelle system.
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Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea; Center for Agricultural Microorganism and Enzyme, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:
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- A new analytical method was developed using HPLC to effectively separate tricapryloylglycerol and its breakdown products, achieving impressive resolution and consistency in under 15 minutes.
- An interface-based kinetic model was created to better understand lipase selectivity during the breakdown of medium- and long-chain TAGs, providing improved results compared to previous models and enhancing knowledge for future lipid production.
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