AI Article Synopsis
- Understanding how the arrangement of adhesive ligands affects cell behavior is key for biomaterial design.
- This study examines the effect of different RGD ligand spacings (30 nm vs. 150 nm) on human mesenchymal stromal cells using specialized hydrogels.
- Results showed that smaller spacings encouraged larger cell spreading, while larger spacings led to elongated shapes, with the αβ integrin being crucial in mediating these changes.
- These findings enhance our knowledge of cell-material interactions and can inform the design of biomaterials for better tissue engineering outcomes.
Article Abstract
Understanding how the spatial distribution of adhesive ligands regulates cell behavior is crucial for designing biomaterials. This study investigates how precisely controlled ligand spacing affects cell spreading and integrin subtype engagement. Using engineered polyacrylamide hydrogels with gold nanoparticle arrays, we explored the impact of RGD ligand spacings (30 and 150 nm) on human mesenchymal stromal cells. Cells exhibited distinct morphological behaviors: smaller spacings promoted larger spreading areas, while larger spacings resulted in elongated shapes with reduced spreading. Mechanistically, we found that the αβ integrin, not the αβ integrin, played a central role in mediating these responses, alongside lamellipodia formation. Our findings provide critical insights into the spatial sensing of ligands, highlighting the influence of ligand spacing on cellular mechanotransduction and integrin-specific responses. This work advances the understanding of cell-material interactions and offers potential strategies for designing biomaterials to guide cell behavior in tissue engineering.
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| http://dx.doi.org/10.1021/acs.langmuir.4c02796 | DOI Listing |
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