AI Article Synopsis
- The study investigates the hepatoprotective (liver-protecting) properties of a popular Ayurvedic herb by analyzing its phytochemicals using targeted metabolomics.
- Different fractions of the herb's hydro-alcoholic extract were tested for antioxidant and protective effects on liver cells (HepG2), with the n-butanol fraction showing the best results.
- UPLC-QTOF-MS analysis identified 17 secondary metabolites in the active fraction, some of which may inhibit the CYP2E1 enzyme, potentially reducing toxicity from APAP (acetaminophen) in liver cells.
Article Abstract
is a popular hepatoprotective herb according to 'Ayurveda'. This study characterises the phytochemicals of responsible for hepatoprotective properties was executed using targeted metabolomics approach. Different fractions of hydro-alcoholic extract of were subjected to assess antioxidant and hepatoprotective properties in HepG2 cells. Furthermore, active fraction was further subjected to UPLC-QTOF-MS based targeted metabolomics to identify the phytochemicals linked to bioactivity. A complementary experiment was also performed to understand the interactions of identified molecules with CYP2E1 enzyme. It was observed that, n-butanol fraction deciphers significant ( < .05) and maximum antioxidant and hepatocyte protection compared to other fractions. UPLC-QTOF-HRMS analysis reveals that it contains 17 secondary metabolites various classes. Identified molecules showed potential interactions with the crucial amino acid residues in the active site of CYP2E1 protein indicate the possibility of inhibition which may counter APAP induced toxicity in HepG2 cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/14786419.2024.2426063 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multi-omics in exploring the pathophysiology of diabetic retinopathy.
Front Cell Dev Biol
December 2024
Department of Ophthalmology, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, China.
Diabetic retinopathy (DR) is a leading global cause of vision impairment, with its prevalence increasing alongside the rising rates of diabetes mellitus (DM). Despite the retina's complex structure, the underlying pathology of DR remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) and recent advancements in multi-omics analyses have revolutionized molecular profiling, enabling high-throughput analysis and comprehensive characterization of complex biological systems.
View Article and Find Full Text PDFUnveiling Metabolic Signatures in Osteoarthritis Progression through Non-Targeted Metabolomics Analysis: A Paradigm Shift in Diagnosis and Treatment Prospects.
Phenomics
October 2024
Department of Orthopedics, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 200072 Shanghai, P. R. China.
BC99 alleviates hyperuricemia and oxidative stress via DAF-16/SKN-1 activation in .
Front Microbiol
December 2024
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, China.
Introduction: Hyperuricemia (HUA) refers to the presence of excess uric acid (UA) in the blood, which increases the risk of chronic kidney disease and gout. Probiotics have the potential to alleviate HUA.
Methods: This study established a hyperuricemia model using (), and studied the anti-hyperuricemia activity and potential mechanisms of BC99 () at different concentrations (10 CFU/mL BC99, 10 CFU/mL BC99).
Network Pharmacology and Metabolomics Reveal Anti-Ferroptotic Effects of Curcumin in Acute Kidney Injury.
Drug Des Devel Ther
December 2024
Research Center of Innovation, Entrepreneurship, Minjiang University, Fuzhou, 350100, People's Republic of China.
Introduction: Acute kidney injury (AKI) is linked to high rates of mortality and morbidity worldwide thereby posing a major public health problem. Evidences suggest that ferroptosis is the primary cause of AKI, while inhibition of monoamine oxidase A(MAOA) and 5-hydroxytryptamine were recognized as the defender of ferroptosis. Curcumin (Cur) is a natural polyphenol and the main bioactive compound of , which has been found nephroprotection in AKI.
View Article and Find Full Text PDF[Advances in molecular networking technology for discovering emerging contaminants and transformation products].
Se Pu
January 2025
State Key Laboratory of Pollution Control & Resource Reuse, School of Environment, Nanjing University, Nanjing 210046, China.
Emerging contaminants and their transformation products are widely distributed in the environment. These pollutants carry unknown risks owing to their persistence, migration, and toxicity. The wide variety and complex structures of these substances render them difficult to identify using only target analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!