Utilizing ionic liquids as eco-friendly and sustainable carriers for delivering nucleic acids: A review on the revolutionary advancement in nano delivery systems.

Ionic liquids (ILs) are an extremely versatile class of chemicals. It has been shown that they can effectively pass through many biological barriers in the human body to deliver medications. ILs are solvents noted for their ecological friendliness; they contain equal amounts of cations and anions and remain liquid at temperatures below 100 °C. Hence, these are ideal for biomedical applications owing to their advantageous properties such as biocompatibility, solubility, and adaptability. ILs are widely reported to improve the solubility and stability of nucleic acids (DNA and RNA) in aqueous conditions, allowing for more effective delivery. Certain ILs have shown the ability to enhance the absorption of nucleic acids into cells. In addition, ILs can also be used to create vectors for gene delivery, such as liposomes and nanoparticles, thereby improving the transfection efficiency of plasmid DNA and siRNA. Subsequently, the application of ILs for nucleic acid delivery has increased significantly in recent years. In this context, we believe that using ILs to enhance the transport of nucleic acids will have a considerable effect as a novel and crucial therapeutic method in the upcoming decades. The use of ILs as solvents to preserve the natural structure of DNA and RNA shows promise for a variety of biotechnological and medical applications. Notably, ILs may be utilized for a variety of functions, including extracting, concentrating, stabilizing, and spreading nucleic acids inside cells. Our review emphasizes the key findings of research works published in this domain, wherein outstanding effectiveness of delivering RNA to the desired areas was achieved, and was made possible through the utilization of ILs.