Monolithic gold saturated on nanoporous gold@mirror template for highly reliable and fast surface-enhanced Raman scattering detection.

The primary challenge hindering the broad application of surface-enhanced Raman scattering (SERS) is the variability in substrate performance due to site differences, leading to unstable detection results. Thus, the current work reports the constant potential deposition of gold (Au) nanostructure on a hybrid nanoporous gold (npAu)-Au mirror template to generate highly stable monolithic Au-saturated npAu@Au-mirror substrate. By systematically adjusting electrochemical variables, different sizes, shapes, and nanogaps of Au nanostructure are generated with high-intensity electromagnetic field regions (hot junctions) for enhanced SERS response. The resulting reproducible and enhanced SERS response of the Au-saturated npAu@Au-mirror mainly originates from i) the precise control of potential and accumulation time (-0.3 V and t = 100 s) to generate uniform and symmetric particle size, dispersion, and nanogaps formation through the reduction, nucleation, and growth of Au nanoparticles; ii) free selective dealloying and complete immersion of AuAg to ensure identical conditions across the alloy surface. The metrics for substrate efficiency were evaluated for reproducibility (n = 100, RSD = 14.22%), signal stability (storage time 25 days and variation in batches and multiple synthesis runs) and enhancements (enhancement factor∼1.3 × 10). The substrate was extended for the rapid and direct malachite green detection in fish samples with a detection limit of 1.7 × 10 mol L and good recoveries (96.69 ± 2.18%∼98.51 ± 0.63%). The monolithic Au-saturated npAu@Au-mirror substrate might be implemented for routine analysis of other target types in food safety-related applications.