Three-dimensional (3D) display can provide more information than two-dimensional display, and real-time 3D reconstruction of the real-world environment has broad application prospects as a key technology in the field of meta-universe and Internet of Things. 3D holographic display is considered to be an ideal 3D display scheme, thus enhancing the computational speed and reconstruction quality of 3D holograms can offer substantial support for real-time 3D reconstruction. Here, we proposed a real-time 3D holographic photography for real-world scenarios driven by both physical model and artificial intelligence. The 3D information of the real scene was acquired by a depth camera and then divided into 30 layers using the layer-based method. Convolutional neural networks (CNN) were used to build the mapping of intensity and depth maps to computer-generated holograms (CGH). The differentiability of the angular spectrum algorithm was used to realize the self-supervised training of the network, while the composite loss function was employed to optimize network parameters by calculating the loss between reconstructed and target images. The trained network can generate a CGH with a resolution of 1024×1024 in 14.5 ms. The proposed system operates at 22 frames per second and successfully reconstructs 3D video of dynamic scene. The system exhibits significant potential for application in intelligent manufacturing, remote office work, distance education and other fields.
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http://dx.doi.org/10.1364/OE.529107 | DOI Listing |
J Biomed Opt
January 2025
Tel Aviv University, Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv, Israel.
Significance: Imaging flow cytometry allows highly informative multi-point cell analysis for biological assays and medical diagnosis. Rapid processing of the imaged cells during flow allows real-time classification and sorting of the cells. Off-axis holography enables imaging flow cytometry without chemical cell staining but requires digital processing to the optical path delay profile for each frame before the cells can be classified, which slows down the overall processing throughput.
View Article and Find Full Text PDFSci Rep
January 2025
Laboratory for Radiophysical and Optical Methods of Environmental Research, National Research Tomsk State University, Tomsk, Russia, 634050.
Monitoring the parameters and behavior of plankton makes it possible to assess the state of the aquatic ecosystem and detect the beginning of an environmental disaster at an early stage. In this respect, the most informative method for the in situ plankton study is underwater digital holography. This method allows obtaining information on the size, shape, and location of plankton individuals, as well as performing their classification and biotesting according to their behavioral responses using a submersible holographic camera non-invasively, in real time, and in the automatic mode.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian, Liaoning, 116034, China.
Bacterial infection is a crucial factor resulting in public health issues worldwide, often triggering epidemics and even fatalities. The accurate, rapid, and convenient detection of viable bacteria is an effective method for reducing infections and illness outbreaks. Here, an unsupervised learning-assisted and surface acoustic wave-interdigital transducer-driven nano-lens holography biosensing platform is developed for the ultrasensitive and amplification-free detection of viable bacteria.
View Article and Find Full Text PDFJ Biotechnol
January 2025
Technische Universität Berlin, Department of Biotechnology, Chair of Bioprocess Engineering, Berlin, Germany; Aalborg University, Department of Chemistry and Bioscience, Esbjerg, Denmark. Electronic address:
Digital holographic microscopy (DHM) is a label-free analytical technique for the determination of the cells' volume and their cytosolic refractive index. Here, we demonstrate the suitability of DHM for the quantification of total lipid accumulation in the oleaginous yeast Yarrowia lipolytica. Presently, microbial lipids are gaining increasing attention due to their nutritional value in feed and food applications.
View Article and Find Full Text PDFThree-dimensional (3D) display can provide more information than two-dimensional display, and real-time 3D reconstruction of the real-world environment has broad application prospects as a key technology in the field of meta-universe and Internet of Things. 3D holographic display is considered to be an ideal 3D display scheme, thus enhancing the computational speed and reconstruction quality of 3D holograms can offer substantial support for real-time 3D reconstruction. Here, we proposed a real-time 3D holographic photography for real-world scenarios driven by both physical model and artificial intelligence.
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