Objective: We studied the role of LNCRNA 1197 in rheumatoid arthritis (RA) and its underlying mechanism.
Methods: We detected expression of LNCRNA 1197 in RA fibroblast-like synovial cells (RA-FLS) by RT-qPCR. Functional experiments were conducted to assess the impact of LNCRNA on cells as demonstrated by a Transwell assay. In addition, Western blot analysis was conducted to analyze the expression of proliferation-related proteins and flow cytometry and CCK-8 to analyze cell cycle and cell proliferation. We also identified the potential downstream target miRNA of LNCRNA 1197.
Results: LNCRNA 1197 was highly expressed in RA-FLS. When LNCRNA 1197 was knocked down, the cell proliferation, migration, and invasion of RA-FLS were alleviated, and pro-inflammatory cytokines were significantly downregulated. Interestingly, LNCRNA 1197 was indicated to target miR-206 and promoted progression of FLS by inhibiting miR-206.
Conclusion: Taken altogether, LNCRNA 1197 promotes the progression of RA-FLSs by miR-206 inhibition.
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