AI Article Synopsis
- RIDR (Rapid Inducible Decay of RNA) is a method that allows scientists to control the degradation of RNA in cells, helping to study how RNA breaks down over time.
- The protocol enables quick and synchronized decay of target mRNAs, allowing researchers to visualize and analyze RNA degradation processes in live cells.
- Detailed instructions for creating stable cell lines, conducting experiments, and analyzing data are provided, along with tips to avoid common mistakes, making this method accessible for various biology labs.
Article Abstract
Modulating RNA decay is a powerful tool to investigate RNA degradation dynamics. Here, we describe a protocol to inducibly recruit protein factors to regulate target RNA metabolism, called Rapid Inducible Decay of RNA (RIDR). RIDR induces fast and synchronous decay of target mRNAs within minutes and enables direct visualization of mRNA decay dynamics and subcellular kinetics in living cells. Here, we provide detailed procedures to make stable cell lines, conduct fixed- and live-cell measurements, and perform data analysis. We discuss the potential pitfalls and make RIDR applicable to a general biology lab.
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| http://dx.doi.org/10.1007/978-1-0716-4176-7_13 | DOI Listing |
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