Eukaryotic mRNAs carry an N7-methylguanosine (mG) cap structure at their 5' extremity, which protects them from the degradation by 5'-3' exoribonucleases and plays a pivotal role in mRNA metabolism, promoting splicing, nuclear export, and translation. Decapping, the enzymatic process that removes this structure, is a key event during cytoplasmic mRNA 5'-3' decay, leading to the degradation of the transcript body by Xrn1. In this chapter, we describe a procedure to assess the cap status of RNA at the transcriptome level. It is based on a treatment of total RNA extracts with a 5' monophosphate-dependent exonuclease, which like Xrn1 specifically degrades decapped RNAs harboring 5' monophosphate extremities, but not RNAs with intact mG cap. The digested RNAs are then analyzed by RNA sequencing.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-4176-7_9 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development of a territory-wide household-based composite index for measuring relative distribution of households by economic status in individual small areas throughout Hong Kong.
BMC Public Health
December 2024
Social Statistics Division, Census and Statistics Department of the Government of the Hong Kong Special Administrative Region, Wanchai Tower, 12 Harbour Road, Wan Chai, Hong Kong Special Administrative Region, China.
Background: Many countries have developed their country/nation-wide multidimensional area-based index on deprivation or socioeconomic status for resource allocation, service planning and research. However, whether each geographical unit proxied by a single index is sufficiently small to contain a relatively homogeneous population remains questionable. Globally, this is the first study that presents the distribution of domestic households by the territory-wide economic status index decile groups within each of the 2,252 small subunit groups (SSUGs) throughout Hong Kong, with a median study population of 1,300 and a median area of 42,400 m.
View Article and Find Full Text PDFInvestigating the complementary value of OCT to MRI in cognitive impairment in relapsing-remitting multiple sclerosis.
Mult Scler
December 2024
Queen Square MS Centre, Department of Neuroinflammation, UCL Queen Square Institute of Neurology, Faculty of Brain Sciences, University College London, London, UK.
Background: Cognitive decline in multiple sclerosis (MS) is associated with neuro-axonal loss, quantifiable by optical coherence tomography (OCT). Associations between OCT measures and cognition in relapsing-remitting MS (RRMS) remain incompletely investigated, particularly the added value of OCT when combined with magnetic resonance imaging (MRI). We investigated the contributions of OCT and MRI while applying stringent criteria to control for subclinical optic neuropathy.
View Article and Find Full Text PDFAssociation Between Multivitamin Use on Admission and Clinical Outcomes in Patients Hospitalised with Community-Acquired Pneumonia: A Case-Cohort Study.
Nutrients
November 2024
Discipline of Medicine, University of Adelaide, Adelaide 5005, Australia.
Background/objectives: Community-acquired pneumonia (CAP) is a leading cause of hospitalisations worldwide. Micronutrient deficiencies may influence CAP risk and severity, but their impact on CAP outcomes remains unclear. This study investigated the influence of multivitamin use on hospital length of stay (LOS), intensive care unit (ICU) admission, in-hospital mortality, and 30-day readmissions in hospitalised CAP patients.
View Article and Find Full Text PDFCognitive impairment predicts medication discrepancies in Huntington's Disease: patient self-report compared to pharmacy records.
J Neurol
December 2024
Department of Neurology, Leiden University Medical Center, PO 9600, 2300RC, Leiden, The Netherlands.
Article Synopsis
Quick Sequential Organ Failure Assessment (qSOFA) and Performance Status Scoring Systems as Prognostic Predictors in Pneumococcal Community-Acquired Pneumonia.
Cureus
November 2024
Department of Infectious Diseases and Infection Control, Yamagata Prefectural Central Hospital, Yamagata, JPN.
Background and aim , or pneumococcus, is one of the most common pathogens responsible for community-acquired pneumonia (CAP), which can progress to sepsis and lead to severe illness. Several clinical scoring systems are widely used to assess the severity of CAP and sepsis. This study aims to evaluate the clinical factors that predict mortality in pneumococcal CAP (pCAP).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!