AI Article Synopsis
- Recent advances in fluorescence microscopy allow for real-time observation of individual RNA translation dynamics in living cells using multiple colors.
- The described protocol enables simultaneous imaging of two open reading frames on a single reporter RNA that are offset from each other.
- This method provides detailed insights into frameshifting dynamics and efficiency, measured at the single-RNA level, by examining specific frameshift stimulatory sequences.
Article Abstract
Recent advances in fluorescence microscopy have now made it possible to measure the translation dynamics of individual RNA in living cells and in multiple colors. Here we describe a protocol that exploits these recent advances to simultaneously image the translation of two open reading frames encoded on a single reporter RNA yet frameshifted with respect to each other. This enables precise measurements of frameshifting dynamics and efficiency from specific frameshift stimulatory sequences, all with single-RNA precision.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11633442 | PMC |
| http://dx.doi.org/10.1007/978-1-0716-4248-1_9 | DOI Listing |
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