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This study aimed to create a new recombinant virus by modifying the EV-A71 capsid protein, serving as a useful tool and model for studying human Enteroviruses. We developed a new screening method using EV-A71 pseudovirus particles to systematically identify suitable insertion sites and tag types in the VP1 capsid protein. The pseudovirus's infectivity and replication can be assessed by measuring postinfection luciferase signals.

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Somatic DNA Variants in Epilepsy Surgery Brain Samples from Patients with Lesional Epilepsy.

Int J Mol Sci

January 2025

Department of Neuropediatrics, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin, and Berlin Institute of Health, 13353 Berlin, Germany.

Epilepsy affects 50 million people worldwide and is drug-resistant in approximately one-third of cases. Even when a structural lesion is identified as the epileptogenic focus, understanding the underlying genetic causes is crucial to guide both counseling and treatment decisions. Both somatic and germline DNA variants may contribute to the lesion itself and/or influence the severity of symptoms.

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is considered one of the prioritized ESKAPE microorganisms for the research and development of novel treatments by the World Health Organization, especially because of its remarkable persistence and drug resistance. In this review, we describe how this can be acquired by the enzymatic degradation of antibiotics, target site modification, altered membrane permeability, multidrug efflux pumps, and their ability to form biofilms. Also, the evolution of drug resistance in , which is mainly driven by mobile genetic elements, is reported, with particular reference to plasmid-associated resistance, resistance islands, and insertion sequences.

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: Legg-Calvé-Perthes disease (LCPD) presents challenges in treatment due to its varied course and unclear etiology. This study aimed to evaluate the efficacy of combining proximal femoral varus osteotomy (PFVO) with a modified trochanteric flip osteotomy to address biomechanical consequences and improve hip abductor muscle strength. : We present a modified approach combining PFVO with a trochanteric flip osteotomy.

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The sub pronator approach to the coronoid process.

Orthop Traumatol Surg Res

January 2025

Department of Orthopaedic Surgery and Traumatology, Saint-Antoine Hospital, Sorbonne Université, 184, rue du Faubourg-Saint-Antoine, 75012 Paris, France.

Introduction: The aim of our study was to describe a new anteromedial approach that allows exposure of the anteromedial facet of the coronoid process and to characterize the position of the median nerve's motor branches relative to this approach in relation to elbow positioning.

Material And Methods: We performed 16 anteromedial approaches on fresh anatomical specimens. The minimum distance between the medial edge of the trochlea and the second branch of the median nerve was measured in three elbow positions: forearm in supination with the elbow extended, forearm in pronation with the elbow extended, and forearm in supination with the elbow flexed at 90 °.

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