AI Article Synopsis
- Carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a major public health concern due to its role as a superbug causing hospital infections, and this study identifies a new strain called sequence type 6758.
- The researchers conducted antimicrobial susceptibility testing and comprehensive genome analysis using advanced sequencing technologies, finding multiple resistance mechanisms and 16 resistance genes.
- Their findings highlight the widespread prevalence of a specific type of plasmid (IncX3) carrying resistance genes in CRKP strains globally, especially in China, underscoring the need for measures to control the spread of these resistant plasmids.
Article Abstract
Purpose: Carbapenem-resistant (CRKP) has emerged as a significant public health threat, particularly as a superbug responsible for nosocomial infections. In this study, we report a novel sequence type 6758 of harboring the gene.
Material And Methods: Antimicrobial susceptibility testing was conducted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The complete genome sequence of the strain was determined using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Genomic features and resistance mechanisms of the strain were further comprehensively analysed using various bioinformatics approaches.
Results: Antimicrobial susceptibility testing revealed that this strain exhibited resistance to multiple antimicrobials, including ceftazidime, ceftriaxone, cefazolin, cefepime, imipenem, meropenem, ampicillin/sulbactam, and sulfamethoxazole/trimethoprim. The genome analysis identified sixteen resistance genes. The carbapenemase gene is located on a 47,823 bp IncX3-type plasmid (pNDM-CRKP331). A total of 41 strains carrying similar IncX3-type plasmids were retrieved from the NCBI database, representing 20 sequence types (STs) across 11 countries. The most common resistance gene carried by these IncX3-type plasmids is , and all these plasmids contain only the gene. The -carrying IncX3-type plasmids are widely prevalent in in China, spanning 15 STs.
Conclusion: In summary, our study reports the first genome sequence of an ST 6758 strain containing the class B β-lactamase isolated from a clinical sample. Given the global emergence of , measures should be taken to prevent the spread of these -carrying IncX3-type plasmids. Our findings contribute to the understanding of the transmission mechanisms of in .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556245 | PMC |
| http://dx.doi.org/10.2147/IDR.S488223 | DOI Listing |
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