Rotavirus-specific-IgA and cytokines responses in infected preschool-aged Nigerian children following rotavirus vaccination.

Rotavirus diarrhea and () infection increase intestinal morbidity and were associated with altered immune responses that compromise the vaccine efficacy in children. The serum level of rotavirus specific IgA (RV-IgA) and cytokine profiles in () infected preschool-aged Nigerian children were estimated following oral rotavirus vaccination. Nineteen of the 149 preschool-aged children (aged 6 to 60 months) with infection paired with age and sex-matched helminth - free children were administered with oral rotavirus vaccine after intestinal helminth screening using stool sample concentration technique. Separated sera from 3 mL venous blood samples were collected and estimated for cytokines (IFN-γ, TNF-α, IL-4, IL-8 IL-6, IL-10) and RV-IgA before and three weeks after rotavirus vaccination using Enzyme Linked Immunosorbent Assay. IFN-γ, IL-8, IL-4 were significantly lower at post-vaccination in -infected children compared with pre-vaccination. Serum IL-10 was significantly higher at post-vaccination in both -infected children and helminth-free controls, compared with pre-vaccination levels ( < 0.05). Pre-vaccination IL-8 and IL-6 were significantly higher in -infected children, while the post-vaccination IL-8 was significantly higher in -infected compared with control. At post-vaccination period, RV-IgA level was lower in -infected children and significantly higher in helminth - free control group compared to pre-vaccination RV-IgA level. infection contributed to down-regulation of some cytokines and antibody responses to oral rotavirus vaccine.