AI Article Synopsis
- Glutamine cyclase, linked to posttranslational modifications, is encoded by the QPCT gene, which shows higher expression levels in HBV-infected HepG2.2.15 cells compared to non-infected HepG2 cells.
- A significant increase in QPCT serum levels was found in HBV-infected patients relative to healthy controls, suggesting its potential role as a biomarker.
- The study indicates that HBV infection enhances QPCT gene expression, which further promotes HBV replication and expression in various cell lines.
Article Abstract
Glutamine cyclase, an enzyme involved in posttranslational modifications, is encoded by the glutaminyl-peptide cyclotransferase (QPCT) gene. Gene microarray analysis revealed that the QPCT gene was highly expressed in HepG2.2.15 cells compared with that in HepG2 cells. The serum expression level of the QPCT gene was detected by ELISA and was significantly greater in HBV-infected patients than in healthy controls. The mRNA and protein expression levels of the QPCT gene were markedly greater in the HBV-expressing cell lines (HepG2.2.15, and HepG2 and Huh7 cells transfected with the pBlu-HBV plasmid) than in the HepG2 and Huh7 cells. The levels of HBV pgRNA and HBV-DNA copy number, as well as the levels of HBeAg and HBsAg, also increased in the HepG2 and Huh7 cell lines cotransfected with the QPCT gene expression plasmid and the HBV 1.3-fold plasmid. Our study indicated that HBV can promote the expression of the QPCT gene, which in turn promotes the expression and replication of HBV.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11556691 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0312773 | PLOS |
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