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Nanosilver-Enhanced Far-Field Fluorescence Fluctuations for Super-Resolution Microscopy. | LitMetric

AI Article Synopsis

  • Fluctuation-based super-resolution microscopy improves image resolution but is limited by the natural fluctuations of fluorophores, which impacts spatiotemporal resolution.
  • A nanosilver film introduces a far-field enhancement (FFE) effect that amplifies fluorescence fluctuations, even from fluorophores located 10 μm away, enhancing the imaging capabilities.
  • The newly developed far-field enhanced super-resolution microscopy (FFE-SRM) improves temporal resolution by up to 10 times and spatial resolution by about 2 times compared to several existing methods, showcasing its effectiveness in live-cell imaging of mitochondrial dynamics.

Article Abstract

Fluctuation-based super-resolution microscopy enhances image resolution using signal fluctuations, yet the inherent fluctuations of fluorophores limit its spatiotemporal resolution. In this work, we reveal a far-field enhancement (FFE) effect via a nanosilver film that significantly boosts fluorescence fluctuations of fluorophores positioned up to 10 μm away. The FFE effect arises from the interference of scattered light from the nanosilver film and photothermal-induced refractive index changes in the imaging medium, which create periodic auxiliary illumination on the sample. This phenomenon enabled the development of far-field enhanced super-resolution microscopy (FFE-SRM), a technique compatible with commonly used fluorophores. FFE-SRM improves temporal resolution up to 10-fold and enhances spatial resolution by about 2-fold over various SRM methods, including stochastic optical fluctuation imaging, super-resolution radial fluctuation, mean-shift super-resolution, and direct stochastic optical reconstruction microscopy. We demonstrated the potential of FFE-SRM by revealing mitochondrial dynamics in live-cell imaging, advancing super-resolution imaging, and cellular process exploration.

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Source
http://dx.doi.org/10.1021/acs.nanolett.4c05155DOI Listing

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