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The chemoreceptor controlling the Wsp-like transduction pathway in Halomonas titanicae KHS3 binds and responds to purine derivatives. | LitMetric

AI Article Synopsis

  • The marine bacterium Halomonas titanicae KHS3 has a chemosensory pathway called HtChe2 that activates a diguanylate cyclase, altering colony appearance and enhancing biofilm formation, similar to the Wsp pathway in Pseudomonas.
  • Research focused on Htc10, the only chemoreceptor in the HtChe2 system, revealing that it binds to guanine and hypoxanthine with specific interaction dynamics, which were characterized using advanced techniques like X-ray crystallography.
  • The study found that when Htc10 was expressed in a Pseudomonas putida mutant lacking its own Wsp receptor, biofilm formation increased, especially with Htc10 ligands,

Article Abstract

The chemosensory pathway HtChe2 from the marine bacterium Halomonas titanicae KHS3 controls the activity of a diguanylate cyclase. Constitutive activation of this pathway results in colony morphology alterations and an increased ability to form biofilm. Such characteristics resemble the behavior of the Wsp pathway of Pseudomonas. In this work, we investigate the specificity of Htc10, the only chemoreceptor coded within the HtChe2 gene cluster. The purine derivatives guanine and hypoxanthine were identified as ligands of the recombinantly produced Htc10 ligand-binding domain, with dissociation constants in the micromolar range, and its structure was solved by X-ray protein crystallography. The sensor domain of Htc10 adopts a double Cache folding, with ligands bound to the membrane-distal pocket. A high-resolution structure of the occupied guanine-binding pocket allowed the identification of residues involved in ligand recognition. Such residues were validated by site-directed mutagenesis and isothermal titration calorimetry analyses of the protein variants. Moreover, heterologous expression of Htc10 in a Pseudomonas putida mutant lacking the native Wsp chemoreceptor promoted biofilm formation, a phenotype that was further enhanced by Htc10-specific ligands. To our knowledge, this is the first description of binding specificity of a chemoreceptor that controls the activity of an associated diguanylate cyclase, opening the way for dynamic studies of the signaling behavior of this kind of sensory complex.

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Source
http://dx.doi.org/10.1111/febs.17320DOI Listing

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