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Detection of fungal and bacterial organisms from clinical uterine samples of horses using next-generation sequencing in comparison with culture. | LitMetric

AI Article Synopsis

  • Current methods for detecting pathogens rely mainly on fungal and bacterial cultures, but next-generation sequencing (NGS) shows promise for identifying microbial DNA in equine uterine samples.
  • In the study, there was an 80% agreement for fungal cultures and a 74% agreement for bacterial cultures when compared to NGS, indicating a moderate correlation between the two methods.
  • Overall, next-generation sequencing could serve as a valuable diagnostic tool for better detecting pathogens in equine endometritis, complementing traditional culture methods.

Article Abstract

Fungal and bacterial culture is currently the primary method for pathogen detection and identification. Next-generation sequencing is a powerful method for detecting and identifying the presence of microbial DNA in samples. We evaluated the correlation between fungal and bacterial culture with next-generation sequencing in equine uterine samples. Fungal cultures (n=63) were evaluated based on their culture results. In culture positive (n=16) samples, next-generation sequencing identified the same organism in 10 samples (62.5%), 5 samples did not identify fungal agents, and 1 sample identified other species of fungal agents. In no growth samples (n=42), next-generation sequencing did not identify fungal agents in 37 samples (88.1%), 4 samples had a potential fungal pathogen identified, and one sample identified only non-pathogenic fungal organisms. Fungal culture and next-generation sequencing had an 80% agreement and moderate correlation by Kappa coefficient (0.508). Bacterial culture (n=57) was also evaluated based on bacterial culture results. In bacterial culture Positive samples (n=32), next-generation sequencing identified the same organism in 25 (78.1%) samples, and identified different organisms from the remaining 7 samples. In bacterial no growth samples (n=14), next-generation sequencing detected bacterial presence in 5 samples, and 9 samples had no bacterial DNA identified. The agreement between bacterial culture and next-generation sequencing was 74% with a moderate correlation by Kappa coefficient (0.46). In conclusion, culture and next-generation sequencing have moderate correlation, and next-generation sequencing has the potential as a diagnostic option for enhancing pathogen detection for equine endometritis.

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Source
http://dx.doi.org/10.1016/j.jevs.2024.105214DOI Listing

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