AI Article Synopsis
- The study focuses on HIV-1 non-structural proteins as potential vaccine targets, particularly examining the prevalent A6 strain in Russia and its regional variants in Amur, Arkhangelsk, Irkutsk, and Murmansk.
- Researchers analyzed 48 blood samples from untreated HIV-infected patients and compared consensus sequences of non-structural proteins across these regions using specialized bioinformatics tools.
- Findings indicate that while there are no differences in the Vpr protein across regions, distinct variations exist in other proteins like Tat, Rev, Vpu, Vif, and Nef, highlighting the importance of understanding these protein variations for future medical approaches, despite the study's limited sample size.
Article Abstract
Introduction: HIV-1 non-structural proteins are promising targets for vaccine development and for creating approaches to personalized medicine. HIV-1 sub-subtype A6 has become the dominating strain in Russia. However, the geographic, economic and demographic characteristics of the country can contribute to the formation of differences between A6 variants circulating in different regions. The aim of the study is a comparative analysis of the consensus sequences of non-structural proteins in A6 variants circulating in the Amur Region, in Arkhangelsk, Irkutsk and Murmansk.
Materials And Methods: 48 whole blood samples obtained from HIV-infected patients without experience of therapy observed at the AIDS Centers in Arkhangelsk, Irkutsk, Murmansk and Amur Region were analyzed. HIV-1 whole-genome nucleotide sequences were obtained and were subtyped. Consensus sequences of sub-subtype A6 variants non-structural proteins for each analyzed region were formed. Furthermore, reference sequences of sub-subtype A6 non-structural proteins were formed based on whole-genome sequences retrieved from the international Los Alamos database. Comparison of consensus sequences and references was performed using the MEGA v.10.2.2 and the PSIPRED programs.
Results: Vif, Vpr and Nef reference sequences have been obtained for HIV-1 sub-subtype A6. There was not difference in consensus sequences of Vpr in different regions. Characteristic features were found for consensus sequences of Tat, Rev, Vpu, Vif and Nef proteins in different regions.
Conclusion: A limitation of the study is a small sample size. Overall, the results demonstrate the existing diversity of non-structural proteins in sub-subtype A6 variants in different regions and indicate the relevance of studying the polymorphism of non-structural proteins of virus variants in different regions.
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| http://dx.doi.org/10.36233/0507-4088-262 | DOI Listing |
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