AI Article Synopsis
- The CRISPR/Cas system is an advanced genome-editing tool that has been refined in recent years for various organisms.
- A new method using CRISPR-Cas9 has been developed specifically for fission yeast, enabling quick genome edits.
- This method produces desired strains efficiently without disrupting the surrounding genome or adding selection markers at the target sites.
Article Abstract
The CRISPR/Cas system is a very powerful genome-editing tool that has been developed over the past decade to optimize genome editing for many organisms. Here, we describe a rapid genome-editing method for fission yeast using the CRISPR-Cas9 system. It allows rapid generation of desired auxotrophic and non-auxotrophic strains without perturbing the local genome content by avoiding the insertion of selection markers at target loci.
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| http://dx.doi.org/10.1007/978-1-0716-4168-2_11 | DOI Listing |
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