Severity: Warning
Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 143
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3098
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Severity: Warning
Message: Attempt to read property "Count" on bool
Filename: helpers/my_audit_helper.php
Line Number: 3100
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3100
Function: _error_handler
File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Antioxidant indices and hemocytes apoptosis in the 6th instar larvae of Hermetia illucens., and their correlation with larval growth were evaluated by exposing larvae to different concentrations of Cu for 1, 3 and 5 generations. Cu accumulated in larval hemolymph showed significant dose-dependent relationship with Cu concentrations in diets within a generation. Larval growth was only promoted after low concentrations of Cu exposure for 1 generation, while seriously affected after high concentrations of Cu exposure. Though total antioxidant capacity activity in larval hemolymph in treatment groups was all higher than that in control, it was increased at lower levels of Cu, while decreased with increasing Cu concentrations at higher levels of Cu exposure. The catalase (CAT) activity and metallothioneins (MTs) levels were also characterized as improved at lower levels of Cu, and inhibited at higher levels of Cu exposure. However, CAT activity and MTs levels at higher Cu treatments were significantly lower than that in control. Apoptosis rate of hemocytes was increased with increasing Cu concentrations. Annexin V - fluorescein isothiocyanate (FITC)/ propidium iodide (PI) staining was in accordance with the results exhibited in flow cytometer. Results from transmission electron microscope and comet assay further confirmed that membrane blebbing, nuclear condensation, and DNA fragmentation were gradually apparent with increasing Cu concentration. All parameters in different generation had similar dose-dependent trends, but the effects were strongest in the fifth generation. This study indicated that at some extent growth of H. illucens were associated with antioxidant responses and apoptosis induced by Cu.
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Source |
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http://dx.doi.org/10.1016/j.cbpc.2024.110074 | DOI Listing |
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