AI Article Synopsis
- Inducing spawning in female catfish is well understood, but stimulating male spermiation and understanding testicular maturation remains challenging, sparking research into gene profile changes during this process.
- The study focused on creating de novo transcriptome assemblies for the Asian catfish (Clarias batrachus) and African catfish (C. gariepinus) to identify genes involved in male reproductive processes, particularly after administering human chorionic gonadotropin (hCG) during their reproductive phase.
- Key findings showed an increase in steroidogenesis-related gene expressions (like star, cyp11b1, cyp17a, and cyp21a) after hCG treatment, along with unique reproduction-related genes differing between the species,
Article Abstract
Induction of spawning in catfish is well demonstrated in females while stimulation of spermiation in males seems difficult. This has led to least understanding of gene profile changes during testicular maturation. Expression of the factors after hormonal induction that control reproductive processes has become an intense research area in fish endocrinology. In view of this, de novo assemblies of testicular transcriptomes of the Asian catfish, Clarias batrachus and the African catfish, C. gariepinus, were performed to identify genes/factors involved in testicular maturation. For testicular induction, human chorionic gonadotropin (hCG) was administered in vivo to both the catfish species during active phase of reproduction. 1,68,071 and 1,26,232 assembled transcripts were obtained from C. batrachus and C. gariepinus testicular transcriptomes and were annotated using different databases. Further, in silico analysis suggested the presence of several transcripts that were involved in steroidogenesis and male reproduction. Comparison of transcriptomes of both species revealed the presence of certain unique genes related to reproduction differentially. Expression profiles after hCG induction in testis showed higher expression of certain steroidogenesis related genes such as star, cyp11b1, cyp17a and cyp21a in both the species. Further, expression levels of crucial factors related to testis, such as dmrt1/2/3, were also found to be significantly higher after gonadotropin induction. However, amh, tgfα and dmrt4/5 expression levels were significantly low. Factors related to male reproduction i.e., tekt1, tekt2, sox9, spag1, spata2 and spata7 were found to be differentially upregulated in hCG treated testicular tissues of both the species. Histology analysis indicated that the gonadotropin induction either short or long term is a better strategy to highlight expression profile changes during testicular maturation in teleost.
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| http://dx.doi.org/10.1016/j.cbd.2024.101349 | DOI Listing |
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