FISH combined with RT-PCR facilitates classification of Chinese adult patients with B-other ALL through improved identification of rearrangement.

ZNF384 gene rearrangements are a distinct subtype of adult B cell acute lymphoblastic leukemia (B-ALL). We screened 46 B-other ALL patients for fusions using fluorescent hybridization (FISH) and reverse transcription-polymerase chain reaction (RT-PCR). Clinical data, treatment response, and minimal residual disease (MRD) status were analyzed. Ten (21.7%) patients were positive (nine by FISH, nine by RT-PCR, eight by both FISH showed atypical signals, including 3' signal gain and 5' signal deletion. was the main fusion partner ( = 5). TAF15::ZNF384, SYNRG::ZNF384, CREBBP::ZNF384, and TCF3::ZNF384 fusions were found in one patient each; one case's partner gene is unknown. One patient was MRD-negative at the end of the first induction, lower than in patients without r. -r incidence matched B-other ALL incidence in Chinese patients. Combined FISH and RT-PCR improved detection. ALL with -r has unique features, and lower MRD-negative response may indicate a negative impact on traditional treatments.