AI Article Synopsis
- A new fluorescence sensor specifically detects l-histidine (His) in water using a 1-Cu complex, implementing a ligand displacement approach for enhanced sensitivity.
- The sensor is initially fluorescent but gets quenched by Cu ions; the presence of His displaces the sensor, restoring its fluorescence quickly within 5 minutes.
- This system exhibits a detection limit of 78 nM, works well in complex biological samples like artificial urine, and can be adapted for portable test strips, making it useful for clinical diagnostics.
Article Abstract
We present a highly sensitive and selective fluorescence "turn-on" sensor for l-histidine (His) detection in aqueous solutions utilizing a 1-Cu complex. This sensing platform employs a fluorescence-based ligand displacement approach, featuring a meso-(N'-acetyl-hydrizide)-based BODIPY derivative (1) complexed with Cu. Initially highly fluorescent, compound 1 is selectively quenched by Cu ions, forming the 1-Cu complex. The high affinity between His and Cu effectively displaces 1 from the complex, restoring fluorescence. The system exhibits rapid response (within 5 minutes), excellent sensitivity (detection limit of 78 nM), operational simplicity, and a large fluorescence "turn-on" signal. It demonstrates remarkable selectivity for His over other amino acids, with maleimide masking cysteine interference. Notably stable in complex biological matrices, the sensor has successfully quantified His in artificial urine samples. Its practical applicability extends to paper-based test strips, offering portability and potential for real-time His monitoring in clinical diagnostics and biological systems.
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| http://dx.doi.org/10.1002/asia.202401182 | DOI Listing |
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