Cassava ( Crantz) is a staple crop in tropical and subtropical regions, valued for its high starch content in roots. Effective genetic transformation and genome editing of cassava require efficient screening methods for transgenic and edited plants. In this study, a visual selection marker system using an R2R3-MYB transcription factor anthocyanin 1 gene ( LOC110667474) from a rubber tree ( Müll. .) has been developed to facilitate the identification of transgenic cassava plants. Transgenic cassava lines expressing accumulated anthocyanins in their leaves, allowing for easy visual identification without the need for destructive assays or specialized equipment. Importantly, the accumulation of anthocyanins did not affect the regeneration or transformation efficiency of cassava. Additionally, the AR-CRISPR/Cas9-gRNA system with the gene as a marker produced gene-edited cassava mutants with purple leaves, demonstrating successful editing. This anthocyanin-based visual reporter (AR) system will provide an effective tool for genetic transformation and genome editing in cassava.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11547100PMC
http://dx.doi.org/10.3390/ijms252111808DOI Listing

Publication Analysis

Top Keywords

genetic transformation
12
transformation genome
12
genome editing
12
editing cassava
12
anthocyanin-based visual
8
visual reporter
8
reporter system
8
cassava
8
transgenic cassava
8
system
4

Similar Publications

Tropical peatlands significantly influence local and global carbon and nitrogen cycles, yet they face growing pressure from anthropogenic activities. Land use changes, such as peatland forests conversion to oil palm plantations, affect the soil microbiome and greenhouse gas (GHG) emissions. However, the temporal dynamics of microbial community changes and their role as GHG indicators are not well understood.

View Article and Find Full Text PDF

Squamous cell carcinomas (SCC) are often preceded by potentially malignant precursor lesions, most of which remain benign. The terminal exhaustion phenotypes of effector T-cells and the accumulation of myeloid-derived suppressor cells (MDSC) have been thoroughly characterized in established SCC. However, it is unclear what precancerous lesions harbor a bona fide high risk for malignant transformation and how precancerous epithelial dysplasia drives the immune system to the point of no return.

View Article and Find Full Text PDF

A Morphospace Exploration Using a General Model of Development Reveals a Basic Set of Morphologies for Early Animal Development and Evolution.

J Exp Zool B Mol Dev Evol

December 2024

Genomics, Bioinformatics and Evolution Group, Departament de Genètica I Microbiologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.

What morphologies are more likely to appear during evolution is a central question in zoology. Here we offer a novel approach to this question based on first developmental principles. We assumed that morphogenesis results from the genetic regulation of cell properties and behaviors (adhesion, contraction, etc.

View Article and Find Full Text PDF

Antimicrobial risk assessment-Aggregating aquatic chemical and resistome emissions.

Water Res

December 2024

Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA27AY, UK; SWING - Department of Built Environment, Oslo Metropolitan Uni., St Olavs Plass, Oslo 0130, Norway. Electronic address:

Urban water systems receive and emit antimicrobial chemicals, resistant bacterial strains, and resistance genes (ARGs), thus representing "antimicrobial hotspots". Currently, regional environmental risk assessment (ERA) is carried out using drug consumption data and threshold concentrations derived based on chemical-specific minimum inhibitory concentration values. A legislative proposal by the European Commission released in 2022 addresses the need to include selected ARGs besides the chemical concentration-based ERAs.

View Article and Find Full Text PDF

A review of deep learning models for the prediction of chromatin interactions with DNA and epigenomic profiles.

Brief Bioinform

November 2024

Agricultural Bioinformatics Key Laboratory of Hubei Province, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan 430070, China.

Advances in three-dimensional (3D) genomics have revealed the spatial characteristics of chromatin interactions in gene expression regulation, which is crucial for understanding molecular mechanisms in biological processes. High-throughput technologies like ChIA-PET, Hi-C, and their derivatives methods have greatly enhanced our knowledge of 3D chromatin architecture. However, the chromatin interaction mechanisms remain largely unexplored.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!