A Novel Methylene Blue Indicator-Based Aptasensor for Rapid Detection of .

is a significant opportunistic pathogen highly prevalent in the environment, requiring early detection methods to prevent infections in vulnerable individuals. The most specific aptamer for , F23, has been used for the development of various assays and sensors for early diagnosis and monitoring. In this study, a novel F23-based electrochemical aptasensor was designed using disposal gold screen-printed electrodes (Au-SPEs) with high reproducibility. Methylene blue (MB) was used as an exogenous indicator, which significantly amplified the electrochemical signal and improved the sensitivity of detection. The aptasensor explored a limit of detection (LOD) of 8 CFU·mL and high selectivity for over other interfering bacteria. Furthermore, it showed potential to detect in tap water samples, offering a point-of-care tool for rapidly controlling the growth of this bacterium in various applications.