Int J Mol Sci
Department of Physiology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary.
Published: October 2024
Several ion currents in the mammalian ventricular myocardium are substantially regulated by the sympathetic nervous system via β-adrenergic receptor activation, including the slow delayed rectifier K current and the L-type calcium current. This study investigated the downstream mechanisms of β-adrenergic receptor stimulation by isoproterenol (ISO) on the inward rectifier (I) and the rapid delayed rectifier (I) K currents using action potential voltage clamp (APVC) and conventional voltage clamp techniques in isolated canine left ventricular cardiomyocytes. I and I were dissected by 50 µM BaCl and 1 µM E-4031, respectively. Acute application of 10 nM ISO significantly increased I under the plateau phase of the action potential (0-+20 mV) using APVC, and similar results were obtained with conventional voltage clamp. However, β-adrenergic receptor stimulation did not affect the peak current density flowing during terminal repolarization or the overall I integral. The ISO-induced enhancement of I was blocked by the calcium/calmodulin kinase II (CaMKII) inhibitor KN-93 (1 µM) but not by the protein kinase A inhibitor H-89 (3 µM). Neither KN-93 nor H-89 affected the I density under baseline conditions (in the absence of ISO). In contrast, parameters of the I current were not affected by β-adrenergic receptor stimulation with ISO. These findings suggest that sympathetic activation enhances I in canine left ventricular cells through the CaMKII pathway, while I remains unaffected under the experimental conditions used.
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http://dx.doi.org/10.3390/ijms252111609 | DOI Listing |
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