Genome Analysis of Gene Family and Function Exploration of in L.

The cyclic nucleotide-gated ion channel (CNGC), as a non-selective cation channel, plays a pivotal role in plant growth and stress response. A systematic analysis and identification of the gene family in is crucial for uncovering its biological functions and potential applications in plant science. In this study, we identified 61 members in the genome, which are phylogenetically similar to and can be classified into Groups I-IV (with Group IV further subdivided into IV-a and IV-b). Collinearity analysis with other species provided insights into the evolution of . By homology modeling, we predicted the three-dimensional structure of BnCNGC proteins and analyzed -acting elements in their promoters, revealing diverse roles in hormone regulation, growth, and stress response. Notably, overexpression of () significantly increased seed size, possibly through regulating cell proliferation via the MAPK signaling pathway. Our findings contribute to a better understanding of the gene family and highlight the potential regulatory role of in the seed development of .