Auto Aligning, Error-Compensated Broadband Collimated Transmission Spectroscopy.

Broadband spectral measurements of the ballistic transmission of scattering samples are challenging. The presented work shows an approach that includes a broadband system and an automated adjustment unit for compensation of angular distortions caused by non-plane-parallel samples. The limits of the system in terms of optimal transmission and detected forward scattering influenced by the scattering phase function are investigated. We built and validated a setup that measures the collimated transmission signal in a spectral range from 300 nm to 2150 nm. The system was validated using polystyrene spheres and Mie calculations. The limits of the system in terms of optimal transmission and detected forward scattering were researched. The optimal working parameters of the system, analyzed by simulations using the Monte Carlo method, show that the transmission should be larger than 10% and less than 90% to allow for a reliable measurement with acceptable errors caused by noise and systematic errors of the system. The optimal transmission range is between 25% and 50%. We show that the phase function is important when considering the accuracy of the measurement. For strongly forward-scattering samples, errors of up to 80% can be observed, even for a very small numerical aperture of 6.6·10-4, as used in our experimental system. We also show that errors increase with optical thickness as the ballistic transmission decreases and the multiscattered fraction increases. In addition, errors caused by multiple reflections in the sample layer were analyzed and also classified as relevant for classical absorption spectroscopy.