Genome-wide identification of the Dof gene family: How it plays a part in mediating cold stress response in Prunus mume.

DNA binding with a finger transcription factor (Dof) takes part in several plant physiological activities such as seed germination, flowering time, cold and drought resistance. Although the function, molecular phylogeny and expression pattern of Dof genes in Prunus mume was not clear yet. Here, the gene structure, motif, chromosome location and phylogenetic relationship of the Dof gene family in Prunus species was explored. We identified 24 members of the Dof gene family from P. mume, which were divided into 3 different subgroups. All these PmDof genes can be mapped to the pseudochromosome. Only one pair of tandem duplication genes are located in Chr3, whereas 8 pairs of segmentally duplicated PmDof genes located in Chr1, Chr2, Chr4, Chr5, and Chr7. Motif and gene structure analysis showed that each group had a similar conservative motif and similar exon/intron composition. Cis-acting elements analysis indicate that PmDofs may be involved in regulating abiotic stress response. Gene expression patterns showed that most PmDofs genes were specifically expressed in different tissues and at different stages. We next found that PmDofs genes display an obvious expression preference or specificity in cold stress response according to qRT-PCR analysis. We further observe a great cold resistance in PmDof10/11/20 OE lines, they showed lower electrolyte leakage rate, MDA content and higher soluble sugar/protein, POD/SOD/proline content than WT after -5 °C 6h freezing treatment. This research offers fresh perspectives on the development of PmDofs, enhancing our comprehension of the structure and role of plant Dof gene families.