AI Article Synopsis
- Mitochondrial viscosity is identified as a key biomarker for various diseases, including cancer and neurodegenerative disorders, but measuring it in cells is challenging.
- This study developed three iridium(III) complexes (Ir1-Ir3) with 5-fluorouracil derivatives, focusing on Ir1 which effectively induced cell death in HeLa cells through increased reactive oxygen species, leading to mitochondrial dysfunction.
- The fluorescence lifetime of Ir1 was highly sensitive to changes in cellular viscosity, allowing for advanced imaging techniques to monitor these viscosity changes in real-time during apoptosis, suggesting potential therapeutic and diagnostic uses.
Article Abstract
Mitochondrial viscosity has emerged as a promising biomarker for diseases such as cancer and neurodegenerative disorders, yet accurately measuring viscosity at the subcellular level remains a significant challenge. In this study, we synthesized and characterized three cyclometalated iridium(III) complexes (Ir1-Ir3) containing 5-fluorouracil derivatives as ligands. Among these, Ir1 selectively induced apoptosis in HeLa cells by increasing mitochondrial production of reactive oxygen species (ROS), which triggered a cascade of events leading to mitochondrial dysfunction. Additionally, the fluorescence lifetime of Ir1 demonstrated high sensitivity to intracellular viscosity changes, enabling real-time fluorescence lifetime imaging microscopy (FLIM) of cellular micro-viscosity during apoptosis. These findings underscore the potential of cyclometalated Ir(III) complexes for both therapeutic and diagnostic applications at the subcellular level.
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| http://dx.doi.org/10.1002/cbic.202400756 | DOI Listing |
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