Wavelet Phase Coherence Analysis of Oxyhemoglobin and DeoxyHemoglobin Oscillations to Investigate the Relationship Between Cups of Cupping Therapy.

Research has not demonstrated whether multiple cups of negative pressure cupping therapy would induce interactions of hemodynamic responses between different areas. A multichannel near-infrared spectroscopy (NIRS) was used to assess oxyhemoglobin and deoxyhemoglobin oscillations in response to cupping therapy. Wavelet transform and wavelet phase (WPC) coherence were used to quantify NIRS signals. Three levels of negative pressure (-75, -225, and -300 mmHg) were applied to the gastrocnemius in 12 healthy adults. Oxyhemoglobin coherence between the two inside-cup areas was higher at -75 mmHg compared to -300 mmHg in both metabolic (WPC = 0.80 ± 0.11 vs. 0.73 ± 0.13) and neurogenic (WPC = 0.70 ± 0.11 vs. 0.60 ± 0.17) controls. Deoxyhemoglobin coherence was also higher at -75 mmHg compared to -300 mmHg in both metabolic (WPC = 0.78 ± 0.11 vs. 0.66 ± 0.14) and neurogenic (WPC = 0.67 ± 0.11 vs. 0.58 ± 0.13) controls. Our study provides first evidence on the interaction of hemodynamic responses between the two cups of cupping therapy using WPC analysis of NIRS signals.