AI Article Synopsis
- Enrofloxacin (ENR) is a widely used synthetic antibiotic in veterinary medicine that helps treat animal diseases and enhance livestock growth, but its overuse can harm the environment and human health due to residual contamination.
- Researchers have created a novel sensor using gold nanoparticles and aptamers (AuNPs-Apt) to specifically detect enrofloxacin residues, with impressive detection capabilities.
- This biosensor demonstrates a broad detection range, high accuracy, quick results, low toxicity, and cost-effectiveness, making it a promising tool for monitoring enrofloxacin in various applications.
Article Abstract
Enrofloxacin (ENR), as a synthetic broad-spectrum antibiotic is widely utilized in veterinary medicine to treat animal diseases and promote livestock growth, it can inhibit bacterial DNA gyrase subunit A, thereby preventing bacterial DNA replication and exerting its antibacterial effect. However, excessive use of enrofloxacin poses significant risks to ecological balance and human health due to residual contamination. We have developed a novel ENR aptamer sensor based on the gold nanoparticles/aptamer (AuNPs-Apt) complexes, in which AuNPs were synthesized via the seed method and functionalized with aptamers. The optical properties, particle size, functional groups and morphology of the AuNPs-Apt probe were characterized by transmission electron microscope, Fourier transform infrared spectrometer and UV-vis spectrophotometer, respectively. The aptamer biosensor can specifically identify enrofloxacin, with a wide detection range (0.05-100g ml) and a good linear relationship (R2=0.99) within the detection range. In addition, the biosensor also has the advantages of short detection time, low biological toxicity, good stability, and low detection cost. Therefore, it shows a great prospect for practical application in the field of detecting enrofloxacin residues.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1088/1361-6528/ad8c4b | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response.
BMC Microbiol
December 2024
College of Agriculture and Forestry, Linyi University, Linyi, 276005, Shandong, China.
Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that is responsible for high mortality, morbidity and severe economic losses to the poultry industry globally, posing a substantial risk to the health of poultry. APEC encounters reactive oxygen species (ROS) during the infection process and thus has evolved antioxidant defense mechanisms to protect against oxidative damage. The imbalance of ROS production and antioxidant defenses is known as oxidative stress, which results in oxidative damage to proteins, lipids and DNA, and even bacterial cell death.
View Article and Find Full Text PDFLeifsonia stereocauli sp. nov., Isolated from Stereocaulon tomentosum Fr. Lichen.
Curr Microbiol
December 2024
Yunnan Institute of Microbiology, Chenggong Campus of Yunnan University, Chenggong District, Kunming, 650500, China.
A Gram-stain-positive, aerobic, yellow-pigmented, catalase-positive, oxidase-positive, non-motile with no flagella and irregularly rod-shaped, denominated strain YIM 134122, was isolated from a Stereocaulon tomentosum Fr. lichen gathered on Baima Snow Mountain in Diqing Tibetan Autonomous Prefecture, Yunnan Province, China. The novel strain grew at pH 6.
View Article and Find Full Text PDFArvimicrobium flavum gen. nov., sp. nov., A Novel Genus in the Family Phyllobacteriaceae Isolated From Forest Soil.
Curr Microbiol
December 2024
Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
During the study of microbial diversity of forest soil in the Republic of Korea, a yellow pigment-producing, Gram-stain-negative, rod-shaped, motile bacterium was isolated and designated as strain 1W2. This strain grew at temperature of 10-37 °C, at pH of 5.0-9.
View Article and Find Full Text PDFMetagenome-validated combined amplicon sequencing and text mining-based annotations for simultaneous profiling of bacteria and fungi: vaginal microbiota and mycobiota in healthy women.
Microbiome
December 2024
Faculty of Medicine, Human Microbiome Research Program, University of Helsinki, Helsinki, Finland.
Background: Amplicon sequencing of kingdom-specific tags such as 16S rRNA gene for bacteria and internal transcribed spacer (ITS) region for fungi are widely used for investigating microbial communities. So far most human studies have focused on bacteria while studies on host-associated fungi in health and disease have only recently started to accumulate. To enable cost-effective parallel analysis of bacterial and fungal communities in human and environmental samples, we developed a method where 16S rRNA gene and ITS1 amplicons were pooled together for a single Illumina MiSeq or HiSeq run and analysed after primer-based segregation.
View Article and Find Full Text PDFTn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean.
Commun Biol
December 2024
Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
The beta-rhizobial strain Paraburkholderia phymatum STM815 is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean (Glycine max [L.] Merr.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!